Critical Roles of Ring Finger Protein RNF8 in Replication Stress Responses
| Autor: | Sui Sui Dong, Jun Huang, Yiqun Deng, Hua Cai, Shirley M.-H. Sy, Junjie Chen, Enoch S.L. Yeung, Gabriel Tsz Mei Lok, Jun Jiang, Michael S.Y. Huen, Jun Wu |
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| Rok vydání: | 2011 |
| Předmět: |
DNA Replication
G2 Phase Cell Survival - drug effects Cell Survival DNA repair Ubiquitin-Protein Ligases DNA Single-Stranded Eukaryotic DNA replication DNA and Chromosomes Biochemistry DNA polymerase delta Cell Line Histones DNA replication factor CDT1 Replication factor C Control of chromosome duplication DNA-Binding Proteins - deficiency - metabolism Humans Hydroxyurea Molecular Biology Replication protein A biology DNA Replication - drug effects - genetics Ubiquitination Cell Biology Molecular biology Cell biology DNA-Binding Proteins Protein Transport Checkpoint Kinase 1 biology.protein Origin recognition complex Rad51 Recombinase Protein Kinases DNA Damage HeLa Cells |
| Zdroj: | Journal of Biological Chemistry. 286:22355-22361 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m111.232041 |
| Popis: | Histone ubiquitylation is emerging as an important protective component in cellular responses to DNA damage. The ubiquitin ligases RNF8 and RNF168 assemble ubiquitin chains onto histone molecules surrounding DNA breaks and facilitate retention of DNA repair proteins. Although RNF8 and RNF168 play important roles in repair of DNA double strand breaks, their requirement for cell protection from replication stress is largely unknown. In this study, we uncovered RNF168-independent roles of RNF8 in repair of replication inhibition-induced DNA damage. We showed that RNF8 depletion, but not RNF168 depletion, hyper-sensitized cells to hydroxyurea and aphidicolin treatment. Consistently, hydroxyurea induced persistent single strand DNA lesions and sustained CHK1 activation in RNF8-depleted cells. In line with strict requirement for RAD51-dependent repair of hydroxyurea-stalled replication forks, RNF8 depletion compromised RAD51 accumulation onto single strand DNA lesions, suggesting that impaired replication fork repair may underlie the enhanced cellular sensitivity to replication arrest observed in RNF8-depleted cells. In total, our study highlights the differential requirement for the ubiquitin ligase RNF8 in facilitating repair of replication stress-associated DNA damage. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc. link_to_subscribed_fulltext |
| Databáze: | OpenAIRE |
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