Bisphenol A affects cell viability involved in autophagy and apoptosis in goat testis sertoli cell
Autor: | Le Han, Hua Yang, Jing Pang, Feng Wang, Yanli Zhang, Guomin Zhang, Fengzhe Li, Peizhen Li |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Male endocrine system Cell Survival Health Toxicology and Mutagenesis Apoptosis 010501 environmental sciences Biology Mitochondrion Endocrine Disruptors Toxicology 01 natural sciences 03 medical and health sciences Bcl-2-associated X protein Phenols Testis medicine Autophagy Animals Viability assay Benzhydryl Compounds Cells Cultured 0105 earth and related environmental sciences bcl-2-Associated X Protein Pharmacology chemistry.chemical_classification Membrane Potential Mitochondrial Reactive oxygen species Sertoli Cells Dose-Response Relationship Drug urogenital system Goats General Medicine Sertoli cell Cell biology Mitochondria Up-Regulation 030104 developmental biology medicine.anatomical_structure chemistry Gene Expression Regulation Proto-Oncogene Proteins c-bcl-2 biology.protein Reactive Oxygen Species hormones hormone substitutes and hormone antagonists Intracellular |
Zdroj: | Environmental toxicology and pharmacology. 55 |
ISSN: | 1872-7077 |
Popis: | Bisphenol A (BPA) is shown to be the endocrine disruptor that induces reproductive dysfunction in male animals. In this study, we aim to probe the effects of BPA exposure on induction of autophagy in goat Sertoli Cells (gSCs), as well as the relationship between autophagy and apoptosis. Results indicated that exposure to BPA (100, 200, 300, 400, 500 and 600μM) decreased the cell viability in a concentration-dependent manner. Exposure of gSCs to 500μM BPA for 12h resulted in in vitro triggered loss of mitochondrial membrane potential (ΔΨm) and increased reactive oxygen species (ROS) production. Apoptosis with an increase in Bax:Bcl-2 ratio and higher rates of autophagy, such as autophagosome formation and increased expression of autophagy-related markers were also induced in gSCs exposed to 500μM BPA. Furthermore, treatment with 350nM Rapamycin (Rap, autophagy activator) alleviated a decrease in cell viability, intracellular ROS production, and reduction of ΔΨm, as well as decreasing apoptosis. Collectively, our results indicated that gSCs viability was disrupted after BPA treatment through affecting ROS production, mitochondrial membrane potential and inducing autophagy/apoptosis. |
Databáze: | OpenAIRE |
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