Mesenchymal Stromal Cells Isolated from Irradiated Human Skin Have Diminished Capacity for Proliferation, Differentiation, Colony Formation, and Paracrine Stimulation

Autor: Vinaya Soundararajan, Charles Wang, Regina Y. Baker, Maxwell B Johnson, Alexander Wong, Sun Y. Park, Xingtian Xu, Xin Chen, Young-Kwon Hong, Brandon Pang, Wei Li, Eunson Jung, Mei Chen, Daniel Gardner, Solmaz Niknam-Bienia
Rok vydání: 2018
Předmět:
0301 basic medicine
Proliferation
Human skin
Stem cells
0302 clinical medicine
Osteogenesis
Neoplasms
Radiation
Ionizing
Migration
Skin
education.field_of_study
lcsh:R5-920
Adipogenesis
integumentary system
lcsh:Cytology
Nuclear Proteins
Cell Differentiation
General Medicine
Endothelial stem cell
Differentiation
Stem cell
lcsh:Medicine (General)
Human
Ionizing radiation
Stromal cell
Mesenchymal
Population
Formins
Wound healing
Biology
03 medical and health sciences
Paracrine signalling
Paracrine Communication
Humans
lcsh:QH573-671
education
Adaptor Proteins
Signal Transducing
Cell Proliferation
Radiotherapy
Mesenchymal stem cell
Mesenchymal Stem Cells
Cell Biology
030104 developmental biology
Cancer research
Transcriptome
030217 neurology & neurosurgery
Developmental Biology
Tissue‐Specific Progenitor and Stem Cells
Zdroj: Stem Cells Translational Medicine
Stem Cells Translational Medicine, Vol 8, Iss 9, Pp 925-934 (2019)
ISSN: 2157-6580
Popis: Ionizing radiation, commonly used in the treatment of solid tumors, has unintended but deleterious effects on overlying skin and is associated with chronic nonhealing wounds. Skin-derived mesenchymal stromal cells (SMSCs) are a pluripotent population of cells that are critically involved in skin homeostasis and wound healing. The aim of this study was to isolate and functionally characterize SMSCs from human skin that was previously irradiated as part of neoadjuvant or adjuvant cancer therapy. To this end, SMSCs were isolated from paired irradiated and nonirradiated human skin samples. Irradiated SMSCs expressed characteristic SMSC markers at lower levels, had disorganized cytoskeletal structure, and had disordered morphology. Functionally, these cells had diminished proliferative capacity and substantial defects in colony-forming capacity and differentiation in vitro. These changes were associated with significant differential expression of genes known to be involved in skin physiology and wound healing. Conditioned media obtained from irradiated SMSCs affected fibroblast but not endothelial cell proliferation and migration. These results suggest that in situ damage to SMSCs during neoadjuvant or adjuvant radiation may play a critical role in the pathogenesis of slow or nonhealing radiation wounds. Stem Cells Translational Medicine 2019;8:925–934
Databáze: OpenAIRE
Externí odkaz: