Histone methyltransferase KMT5A gene modulates oncogenesis and lipid metabolism of papillary thyroid cancer in vitro
| Autor: | Ben Ma, Yuan Jin Wang, Yu Wang, Xiang Ming Mu, Jia Ying Chen, Ning Qu, Rong Liang Shi, Wen Jun Wei, Duan Shu Li, Tian Liao, Guo Hua Sun, Jia Qian Hu, Jun Xiang, Li Tao Han, Qing Guan, Qinghai Ji |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Cancer Research Cell Survival In Vitro Techniques medicine.disease_cause Papillary thyroid cancer Thyroid carcinoma 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Malondialdehyde medicine Humans Thyroid Neoplasms Thyroid cancer Cell Proliferation Neoplasm Staging Chemistry Cancer Lipid metabolism General Medicine Histone-Lysine N-Methyltransferase Cell cycle medicine.disease Lipid Metabolism Carcinoma Papillary 030104 developmental biology Oncology Thyroid Cancer Papillary 030220 oncology & carcinogenesis Histone methyltransferase Gene Knockdown Techniques Lymphatic Metastasis Cancer research Female Carcinogenesis |
| Zdroj: | Oncology reports. 39(5) |
| ISSN: | 1791-2431 |
| Popis: | KMT5A (known as PR-Set7/9, SETD8 and SET8), a member of the SET domain containing methyltransferase family specifically targeting H4K20 for methylation, has been implicated in multiple biological processes. In the present study, we identified that KMT5A was elevated in 50 pairs of papillary thyroid cancer tissue samples and in cell lines K1 and TPC-1 by qRT-PCR and western blotting, as well as by immunohistochemical staining. CCK-8 assay and flow cytometric analysis revealed that inhibition of KMT5A attenuated proliferation and induced apoptosis. Transwell assays revealed that cell migration and invasion were suppressed in KMT5A-knockdown cells. Moreover, the inhibition of KMT5A arrested the cell cycle in the G1/S phase of papillary thyroid cancer cells. The TCGA data revealed that elevated KMT5A expression was significantly correlated with extrathyroidal extension, lymph node metastasis and advanced pathological stage of papillary thyroid cancer. Furthermore, we observed that inhibition of KMT5A suppressed the expression of SREBP1, SCD, FASN and ACC, key molecules involved in lipid metabolism and decreased the level of malondialdehyde in papillary thyroid cancer cells. In conclusion, KMT5A may be a novel oncogenic factor, specifically a regulator for lipid metabolism in papillary thyroid carcinoma. |
| Databáze: | OpenAIRE |
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