Synthetic Polymers Provide a Robust Substrate for Functional Neuron Culture
| Autor: | Mark Bradley, Guy Shpak, Suvra Nath, Yichuan Zhang, Jeffrey Matray, Lidy E. Fratila-Apachitei, Chris I. De Zeeuw, Amir A. Zadpoor, Steven A. Kushner, Seshasailam Venkateswaran, Gustavo A. Higuera |
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| Přispěvatelé: | Netherlands Institute for Neuroscience (NIN), Neurosciences, Psychiatry |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
synthetic polymer substrates
Polymers Cell Biomedical Engineering neuron cultures Pharmaceutical Science 02 engineering and technology 010402 general chemistry 01 natural sciences Biomaterials Extracellular matrix polymer microarrays Mice Neural Stem Cells Laminin medicine Animals central nervous system regeneration Progenitor cell Primary cell Cells Cultured Neurons biology Chemistry Substrate (chemistry) Cell Differentiation 021001 nanoscience & nanotechnology In vitro 0104 chemical sciences Cell biology medicine.anatomical_structure progenitor cell maturation biology.protein Neuron 0210 nano-technology |
| Zdroj: | Advanced Healthcare Materials, 9(4) Zhang, Y, Venkateswaran, S, Higuera, G A, Nath, S, Shpak, G, Matray, J, Fratila-apachitei, L E, Zadpoor, A A, Kushner, S A, Bradley, M & De Zeeuw, C I 2020, ' Synthetic Polymers Provide a Robust Substrate for Functional Neuron Culture ', Advanced Healthcare Materials, pp. 1901347 . https://doi.org/10.1002/adhm.201901347 Advanced healthcare materials, 9. John Wiley and Sons Ltd Advanced healthcare materials, 9(4):e1901347. John Wiley & Sons Ltd. |
| ISSN: | 2192-2640 |
| Popis: | Substrates for neuron culture and implantation are required to be both biocompatible and display surface compositions that support cell attachment, growth, differentiation, and neural activity. Laminin, a naturally occurring extracellular matrix protein is the most widely used substrate for neuron culture and fulfills some of these requirements, however, it is expensive, unstable (compared to synthetic materials), and prone to batch-to-batch variation. This study uses a high-throughput polymer screening approach to identify synthetic polymers that supports the in vitro culture of primary mouse cerebellar neurons. This allows the identification of materials that enable primary cell attachment with high viability even under “serum-free” conditions, with materials that support both primary cells and neural progenitor cell attachment with high levels of neuronal biomarker expression, while promoting progenitor cell maturation to neurons. |
| Databáze: | OpenAIRE |
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