Nuclear factor I genomic binding associates with chromatin boundaries
| Autor: | Giovanna Ambrosini, Jan Kerschgens, A. Gaussin, Giovani Dietler, Jozef Adamcik, Nicolas Mermod, Christoph D. Schmid, Philipp Bucher, Genta Plasari, Petar Pjanic, Milos Pjanic |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
DNA Replication
Transcriptional Activation CAAT box Epigenesis Genetic Mice Genetics Animals Nucleosome Chromatin domain boundaries Promoter Regions Genetic NFI Transcription Factors Binding Sites Genome biology Nuclear factor I Histone modifications Chromosome Mapping DNA Methylation Fibroblasts Chromatin Assembly and Disassembly Chromatin immunoprecipitation Chromatin Nucleosomes DNA-Binding Proteins DNA binding site Histone Gene Expression Regulation Chromatin/genetics Chromatin/metabolism DNA Replication/genetics DNA-Binding Proteins/genetics DNA-Binding Proteins/metabolism Fibroblasts/cytology Fibroblasts/metabolism NFI Transcription Factors/genetics NFI Transcription Factors/metabolism Nucleosomes/genetics Nucleosomes/metabolism Transcriptional Activation/genetics biology.protein Research Article Biotechnology |
| Zdroj: | BMC Genomics, vol. 14, pp. 99 BMC Genomics |
| Popis: | Background: The Nuclear Factor I (NFI) family of DNA binding proteins (also called CCAAT box transcription factors or CTF) is involved in both DNA replication and gene expression regulation. Using chromatin immuno-precipitation and high throughput sequencing (ChIP-Seq), we performed a genome-wide mapping of NFI DNA binding sites in primary mouse embryonic fibroblasts. Results: We found that in vivo and in vitro NFI DNA binding specificities are indistinguishable, as in vivo ChIP-Seq NFI binding sites matched predictions based on previously established position weight matrix models of its in vitro binding specificity. Combining ChIP-Seq with mRNA profiling data, we found that NFI preferentially associates with highly expressed genes that it up-regulates, while binding sites were under-represented at expressed but unregulated genes. Genomic binding also correlated with markers of transcribed genes such as histone modifications H3K4me3 and H3K36me3, even outside of annotated transcribed loci, implying NFI in the control of the deposition of these modifications. Positional correlation between + and - strand ChIP-Seq tags revealed that, in contrast to other transcription factors, NFI associates with a nucleosomal length of cleavage-resistant DNA, suggesting an interaction with positioned nucleosomes. In addition, NFI binding prominently occurred at boundaries displaying discontinuities in histone modifications specific of expressed and silent chromatin, such as loci submitted to parental allele-specific imprinted expression. Conclusions: Our data thus suggest that NFI nucleosomal interaction may contribute to the partitioning of distinct chromatin domains and to epigenetic gene expression regulation. NFI ChIP-Seq and input control DNA data were deposited at Gene Expression Omnibus (GEO) repository under accession number GSE15844. Gene expression microarray data for mouse embryonic fibroblasts are on GEO accession number GSE15871. |
| Databáze: | OpenAIRE |
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