Antigenic determinants of influenza virus hemagglutinin. VIII. Topography of the antigenic regions of influenza virus hemagglutinin determined by competitive radioimmunoassay with monoclonal antibodies
| Autor: | A.M. Breschkin, David O. White, J. Ahern |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
medicine.drug_class
Antibody Affinity Radioimmunoassay Hemagglutinin (influenza) Hemagglutinins Viral Biology Hybrid Cells Monoclonal antibody Antibodies Viral Binding Competitive Epitope Virus Antibodies Cell Line Iodine Radioisotopes Epitopes Antigen Virology medicine Animals Avidity Hemagglutination assay Antibodies Monoclonal Hemagglutination Inhibition Tests Molecular biology Rats Influenza A virus biology.protein Binding Sites Antibody Antibody Multiple Myeloma |
| Zdroj: | Virology. 113(1) |
| ISSN: | 0042-6822 |
| Popis: | Several independent hybrid cell lines (“hybridomas”) that produce monoclonal antibodies against the hemagglutinin (HA) of influenza virus A/Memphis/102/72 were derived in this laboratory. The reactivity of each monoclonal antibody against the virus was characterized by radioimmunoassay (RIA), hemagglutination inhibition (HI), neutralization of infectivity, and avidity of binding. Most hybridoma antibodies reacted with the apoprotein of the HA, and several of these displayed HI and neutralizing activity; one monoclone was directed against the “host antigen” (carbohydrate) and had no HI or neutralizing capacity. This panel of monoclonal antibodies was used to study the topography of epitopes on the HA by means of a competitive RIA; the capacity of unlabeled hybridoma antibodies to block the binding of radiolabeled heterologous monoclones was tested. Some pairs of monoclones competed completely and were therefore judged to be binding in the same antigenic region; other pairs bound noncompetitively to physically distinct regions. The results demonstrate the existence of four distinct antigenic regions. One major region contains epitopes recognized by several monoclones, all of which have substantial HI and neutralizing activity. Two other regions are apparently distinct from but not completely remote from this major region. A fourth region is physically unrelated to the others; monoclones binding to this region had no activity in HI or neutralization assays. |
| Databáze: | OpenAIRE |
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