Cloning and characterization of a naturally occurring antisense RNA to human thymidylate synthase mRNA
| Autor: | Bruce J. Dolnick |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Base Sequence
cDNA library Molecular Sequence Data Intron Proteins RNA-dependent RNA polymerase RNA DNA Thymidylate Synthase Biology Blotting Northern Reverse northern blot Polymerase Chain Reaction Molecular biology Antisense RNA Rapid amplification of cDNA ends RNA editing RNA Precursors Tumor Cells Cultured Genetics Humans RNA Antisense RNA Messenger Cloning Molecular Hydro-Lyases |
| Zdroj: | Nucleic Acids Research. 21:1747-1752 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/21.8.1747 |
| Popis: | Based upon reverse transcription and polymerase chain reaction results with human KB cell RNA, a cDNA (i.e., 3'rTS1, 1557 nt) with complementarity to thymidylate synthase mRNA was cloned and sequenced. Northern blot analysis showed that 3'rTS1 corresponded to a cytoplasmic 1.8 kb RNA found in several tumor cell lines. The remaining 5'region of this antisense RNA was cloned by a RACE (Rapid Amplification of cDNA Ends) procedure. A full length cDNA (i.e., rTS, 1811 nt) was generated by splicing 3'rTS1 with RACE-generated cDNA. rTS RNA is likely a mRNA that contains four open reading frames. Based upon sequence analysis of the RACE cDNAs and the rTS cDNA, rTS RNA is likely processed from a gene containing at least six introns. Northern blot analysis indicates rTS RNA is expressed in a variety of human tumor cell lines and an aberrant from is expressed in a methotrexate-cell line. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|