Irradiation with Polychromatic Incoherent Low Energy Radiation of Human Peripheral Blood Mononuclear Cells In Vitro: Effects on Cytokine Production
| Autor: | Giuseppe Acri, E. Ruello, Carlo Sansotta, Francesca Maria Salmeri, Barbara Testagrossa, Lucia Denaro, Sergio Gurgone |
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| Rok vydání: | 2020 |
| Předmět: |
PILER
Health Toxicology and Mutagenesis medicine.medical_treatment lcsh:Medicine Enzyme-Linked Immunosorbent Assay Peripheral blood mononuclear cell Article 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Low energy Immune system medicine Humans Secretion Irradiation Incubation 030304 developmental biology 0303 health sciences Tumor Necrosis Factor-alpha Chemistry lcsh:R Public Health Environmental and Occupational Health Molecular biology In vitro Cytokine Cytokines PBMCs PILER PBMCs Leukocytes Mononuclear Cytokines |
| Zdroj: | International Journal of Environmental Research and Public Health International Journal of Environmental Research and Public Health, Vol 17, Iss 4, p 1233 (2020) Volume 17 Issue 4 |
| ISSN: | 1660-4601 |
| Popis: | (1) Background: Physical stimuli may activate peripheral blood mononuclear cells (PBMCs) to secrete cytokines, which may favor pro-inflammatory responses or trigger reparative phenomena. The purpose of this study is to evaluate the action of Polarized Polychromatic Incoherent Low Energy Radiation (PILER) on human in vitro PBMCs, by detection of the possible effects on cytokine production (2) Methods: isolated PBMCs were irradiated with a PILER lamp at different exposure times, at a distance of 10 cm, before incubation. The supernatants were collected after 24 h and 48 h and cytokines evaluated by ELISA (3) Results: Our results showed a decrease in the levels of pro-inflammatory IL-12p70, IL-17A, IFN-&gamma and TNF-&alpha cytokines, whereas IL-10 and TGF-&beta 1 with regulatory activity increased (4) Conclusions: PILER irradiation affected the cytokine production by isolated PBMCs driving the immune response toward an anti-inflammatory/reparative profile. |
| Databáze: | OpenAIRE |
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