Clinical correlation of variations in the internal transcribed spacer regions of rRNA genes in Pneumocystis carinii f.sp. hominis
| Autor: | Shaoling Jin, John Yi Chung Hsueh, Jens D Lundgren, Jan Hansen, Bettina Lundgren, Thomas Benfield, Jannik Helweg-Larsen, Chao-Hung Lee |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Genetic Markers Male Genotype Opportunistic infection Immunology Biology Bronchoscopies Cohort Studies HIV Seropositivity medicine Immunology and Allergy Humans Prospective Studies Internal transcribed spacer rRNA Operon Aged AIDS-Related Opportunistic Infections Pneumocystis Pneumonia Pneumocystis Genetic Variation Spacer DNA Middle Aged medicine.disease Virology respiratory tract diseases Infectious Diseases Pneumocystis carinii Genetic marker HIV-1 DNA Intergenic Female RRNA Operon |
| Zdroj: | AIDS (London, England). 15(4) |
| ISSN: | 0269-9370 |
| Popis: | Objectives: To analyse the importance of sequence variations in the internal transcribed spacer (ITS) regions 1 and 2 of the nuclear rRNA operon in AIDS patients with Pneumocystis carinii pneumonia (PCP). Design and methods: ITS 1 and 2 genotypes were determined in 162 bronchoalveolar lavage samples from 130 patients participating in a prospective cohort study of PCP. Results: A total of 49 different ITS genotypes were detected. ITS genotype was not associated with the clinical severity or outcome of PCP. In 37 of 162 (23%) samples infection with two or more genotypes was observed. A genotype switch was detected in six of 10 patients (60%) with recurrent episodes of PCP. However, genotype changes were also seen in 10 of 19 patients (53%) who had repeated bronchoscopies within the same episode of PCP. The same ITS type was observed twice in 13 (46%) of the 28 patients with repeat bronchoscopies during single or recurrent episodes of pneumonia, but in only 14 of 81 (17%) randomly selected pairs (P < 0.01). Conclusion: Although the detection of ITS genotypes is not a random event, changes in genotype can be detected in a single episode of disease, with 23% of PCP patients being infected with more than one P carinii genotype, thus complicating the use of this locus as a genetic marker to separate new infection from the reactivation of latent infection. ITS genotypes are not associated with the clinical severity of PCP. |
| Databáze: | OpenAIRE |
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