Characterization of the nuclear localization signal of the mouse TET3 protein
| Autor: | Xiaolong Zhou, Xian-Ju Huang, Honglin Liu, Kai Xiong, Yi Wang, Yun Teng, Rui Cao, Peng Xiao, Hong-xiao Zhang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Molecular Sequence Data
Nuclear Localization Signals Active Transport Cell Nucleus Biophysics Importin Biology medicine.disease_cause Biochemistry Dioxygenases Green fluorescent protein Mice Proto-Oncogene Proteins medicine Animals NLS Amino Acid Sequence Nuclear protein Molecular Biology Cell Nucleus Mutation Cell Biology DNA-Binding Proteins DNA demethylation NIH 3T3 Cells biology.protein Demethylase Sequence Alignment Nuclear localization sequence |
| Zdroj: | Biochemical and Biophysical Research Communications. 439:373-377 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2013.08.075 |
| Popis: | DNA demethylation is associated with gene activation and is mediated by a family of ten-eleven translocation (TET) dioxygenase. The TET3 protein is a 1668-amino-acid DNA demethylase that is predicted to possess five nuclear localization signals (NLSs). In this paper, we used a series of green fluorescent protein-tagged and mutation constructs to identify a conserved NLS (KKRK) embedded between amino acid 1615 and 1618 of mouse TET3. The KKRK sequence facilitates the cytoplasmic protein's translocation into the nucleus. Additionally TET3 may be imported into the nucleus by importin-α and importin-β. |
| Databáze: | OpenAIRE |
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