Replicative senescence is distinguishable from DNA damage-induced senescence by increased methylation of promoter of rDNA and reduced expression of rRNA
| Autor: | Shin Akakura, Siamak Tabibzadeh, Elena A. Ostrakhovitch, Reiko Sanokawa-Akakura |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Senescence Aging DNA damage Promoter Methylation DNA Methylation Fibroblasts Ribosomal RNA Biology DNA Ribosomal Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Gene Expression Regulation RNA Ribosomal Humans Cellular Senescence 030217 neurology & neurosurgery DNA Damage Developmental Biology |
| Zdroj: | Mechanisms of Ageing and Development. 183:111149 |
| ISSN: | 0047-6374 |
| Popis: | Human fibroblasts become senescent after a limited number of replications or by diverse stresses, such as DNA damage. However, replicative and damage induced senescence are indistinguishable in respect to proliferation cessation and expression of senescence markers, senescence-associated β-galactosidase, p16 and p21. Here, we show that senescence types can be distinguished by reduced levels of 18S, 5.8S and 28S rRNA, in replicative but not induced senescence. We also demonstrate that promoter region of rRNA is hypermethylated in replicative senescence. The findings show that expression level of rRNA or methylation of its promoter can be used to distinguish between senescence types. |
| Databáze: | OpenAIRE |
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