Effect of Spiperone on Mesenchymal Multipotent Stromal and Hemopoietic Stem Cells under Conditions of Pulmonary Fibrosis
| Autor: | I. E. Stepanova, V. M. Reztsova, E. S. Khmelevskaya, D. V. Reikhart, A. M. Dygai, V. A. Krupin, E. G. Skurikhin, O. V. Pershina, A. M. Reztsova, N. N. Ermakova |
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| Rok vydání: | 2014 |
| Předmět: |
Stromal cell
Pulmonary Fibrosis CD34 Gene Expression Bone Marrow Cells General Biochemistry Genetics and Molecular Biology Bleomycin Mice Antigens CD medicine Animals Cell Lineage CD90 Progenitor cell Chemistry Macrophages Mesenchymal stem cell Endothelial Cells Mesenchymal Stem Cells General Medicine Fibroblasts respiratory system Hematopoietic Stem Cells Mice Inbred C57BL Pulmonary Alveoli Haematopoiesis medicine.anatomical_structure Neutrophil Infiltration Spiperone Cancer research Dopamine Antagonists Bone marrow Stem cell Megakaryocytes Biomarkers Granulocytes |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 157:132-137 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-014-2508-y |
| Popis: | The antifibrotic properties of spiperone and its effect on stem and progenitor cells were studied on the model of reversible bleomycin-induced pulmonary fibrosis in C57Bl/6 mice. Spiperone reduced infiltration of the alveolar interstitium and alveolar ducts with inflammatory cells and prevented the growth of the connective tissue in the parenchyma of bleomycin lungs. Apart from anti-inflammatory effect, spiperone suppressed bone marrow hemopoietic cells (CD3, CD45R (B220), Ly6C, Ly6G (Gr1), CD11b (Mac1), TER-119)-, Sca-1+, c-Kit+, CD34- and progenitor hemopoietic cells (granulocyte-erythroid-macrophage-megakaryocytic and granulocyte CFU). Spiperone-induced disturbances of fi brogenesis were paralleled by restoration of endothelial cells in the lung parenchyma, reduction of the number of circulating bone marrow cells and lung mesenchymopoietic cells (mesenchymal multipotent stromal cells (CD31-, CD34-, CD45-, CD44+, CD73+, CD90+, CD106+) and progenitor fi broblast cells), and suppression of multilineage differentiation of multipotent mesenchymal stromal cells (including fi broblast-lineage cells). |
| Databáze: | OpenAIRE |
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