Anti-Ulcerogenic Effect of a Whey Protein Isolate and Collagen Hydrolysates Against Ethanol Ulcerative Lesions on Oral Administration to Rats
| Autor: | A. Possenti, Sirlene Valério Tinti, G.A. Castro, Valdemiro Carlos Sgarbieri, João Carvalho |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Whey protein Alkylation Swine Carbenoxolone Medicine (miscellaneous) Pharmacology Hydrolysate Whey protein isolate chemistry.chemical_compound Oral administration In vivo Gastrins Animals Medicine Stomach Ulcer Sulfhydryl Compounds Rats Wistar Gastrin Nutrition and Dietetics Ethanol biology business.industry Hydrolysis Stomach Drug Synergism Anti-Ulcer Agents Milk Proteins Rats Disease Models Animal Mucus Whey Proteins chemistry Biochemistry Ethylmaleimide Gastric Mucosa biology.protein Cattle Drug Therapy Combination Collagen business medicine.drug |
| Zdroj: | Journal of Medicinal Food. 13:83-90 |
| ISSN: | 1557-7600 1096-620X |
| Popis: | The effect of the administration of a whey protein isolate (WPI) and collagen hydrolysates on ethanol-induced ulcerative lesions was studied in rats. WPI and bovine or porcine collagen hydrolysate (BCH and PCH, respectively) were given to rats by gavage. In acute experiments, (single-dose) physiological saline (10 mL/kg of body weight) was used as the negative control, and carbenoxolone (200 mg/kg of body weight) was used as a positive control. Ethanol (1 mL per 250-g rat) was also given by gavage. These treatments reduced the ulcerative lesion index (ULI) in a range of 40-77%, depending on the dosage. Some mixtures of WPI with either PCH or BCH provided results that suggested synergisms between WPI and the collagen hydrolysates. For example, WPI/BCH (in the proportion of 375:375 mg/kg of body weight) decreased ULI by 64%. The mechanism for mucosal protection involved a decrease in plasma gastrin (approximately 40%), a significant increase (50-267%) in mucus production, and a reduction in ULI (percentage) when intragastric administrations were performed after in vivo alkylation by N-ethylmaleimide. Results suggest that gastrin, sulfhydryl substances, and some mechanisms related to mucus production are all involved in gastric ulcer protection against ethanol. The collagen hydrolysates (both PCH and BCH) presented a stronger effect on mucus production; on the other hand, the effect of WPI was also dependent on sulfhydryl compounds, resulting in a more protective effect when the two proteins were administered together. |
| Databáze: | OpenAIRE |
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