The Effects of Urethane on Rat Outer Hair Cells
| Autor: | Mengzi Chen, Mingyu Fu, Xueying Yang, Jie Tang, Jinfang Xiao, Xiao Yan |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Patch-Clamp Techniques Article Subject Urethane Membrane Potentials lcsh:RC321-571 Rats Sprague-Dawley Motor protein Hair Cells Vestibular 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine otorhinolaryngologic diseases medicine Animals Prestin lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Cochlea Membrane potential biology Strychnine Anatomy Hair Cells Auditory Outer Nicotinic acetylcholine receptor 030104 developmental biology medicine.anatomical_structure Neurology chemistry Anesthetic biology.protein Biophysics sense organs Neurology (clinical) Hair cell 030217 neurology & neurosurgery Research Article medicine.drug |
| Zdroj: | Neural Plasticity, Vol 2016 (2016) Neural Plasticity |
| ISSN: | 1687-5443 2090-5904 |
| DOI: | 10.1155/2016/3512098 |
| Popis: | The cochlea converts sound vibration into electrical impulses and amplifies the low-level sound signal. Urethane, a widely used anesthetic in animal research, has been shown to reduce the neural responses to auditory stimuli. However, the effects of urethane on cochlea, especially on the function of outer hair cells, remain largely unknown. In the present study, we compared the cochlear microphonic responses between awake and urethane-anesthetized rats. The results revealed that the amplitude of the cochlear microphonic was decreased by urethane, resulting in an increase in the threshold at all of the sound frequencies examined. To deduce the possible mechanism underlying the urethane-induced decrease in cochlear sensitivity, we examined the electrical response properties of isolated outer hair cells using whole-cell patch-clamp recording. We found that urethane hyperpolarizes the outer hair cell membrane potential in a dose-dependent manner and elicits larger outward current. This urethane-induced outward current was blocked by strychnine, an antagonist of theα9 subunit of the nicotinic acetylcholine receptor. Meanwhile, the function of the outer hair cell motor protein, prestin, was not affected. These results suggest that urethane anesthesia is expected to decrease the responses of outer hair cells, whereas the frequency selectivity of cochlea remains unchanged. |
| Databáze: | OpenAIRE |
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