Characterization of oral fibroblasts: An in vitro model for oral fibrosis
| Autor: | Alan Mathew Punnoose, Prabhu Manickam Natarajan, Prathibha Prasad, Malathi Narasimhan, Pooja Narain Adtani, Kannan Ranganathan |
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| Rok vydání: | 2019 |
| Předmět: |
transforming growth factor
Pathology medicine.medical_specialty Vimentin Pathology and Forensic Medicine Malignant transformation Masson's trichrome stain Extracellular matrix 03 medical and health sciences 0302 clinical medicine Fibrosis Medicine oral fibroblasts phalloidin Oral mucosa Fibroblast General Dentistry biology business.industry 030206 dentistry medicine.disease medicine.anatomical_structure Otorhinolaryngology Oral submucous fibrosis 030220 oncology & carcinogenesis biology.protein Original Article business |
| Zdroj: | Journal of Oral and Maxillofacial Pathology : JOMFP BASE-Bielefeld Academic Search Engine |
| ISSN: | 0973-029X |
| Popis: | Background: Oral submucous fibrosis (OSMF) is a chronic debilitating condition of the oral mucosa that has been classified as a potentially malignant disorder with a malignant transformation rate of 2%–8%. Several in vitro and in vivo experiments have been performed to formulate a treatment modality for OSMF, yet no ideal in vitro primary oral fibroblast model has been developed. Aim: To establish an in vitro primary oral fibroblast model. Setting and Design: In vitro laboratory setting. Materials and Methodology: Primary cell culture protocol was performed after obtaining normal oral tissue. Karyotyping was performed to rule out chromosomal abnormalities. Immunofluorescence staining was carried with a panel of fibroblast-specific markers (vimentin, phalloidin, transforming growth factor-β receptor 1 [TGFβR1] and s100a4) and Masson trichrome staining (MTS) to demonstrate the presence of extracellular matrix (ECM) qualitatively. Results: A monolayer of oral fibroblasts was observed on the 9th-day postseeding. No chromosomal abnormality was observed in the patient samples. Positive staining was observed with vimentin, phalloidin, TGFβR1 and s100a4, thereby confirming the cell type. MTS revealed fibroblasts with spindle morphology and scanty ECM. Conclusion: The present study lays down a protocol to design and characterize primary buccal fibroblast cell culture model that would aid researchers in performing in vitro preliminary experiments in areas concerning fibrosis. |
| Databáze: | OpenAIRE |
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