Type IV Collagen in the Basement Membrane of the Corneal Epithelium after Alkali Burns in Guinea Pigs
| Autor: | Sakae Kobata, Natsuko Hashizume, Yuka Okada, Shizuya Saika, Osamu Yamanaka, Akira Ooshima |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
Pathology medicine.medical_specialty genetic structures Guinea Pigs Alkali burn Basement Membrane Epithelium Cornea Immunoenzyme Techniques Guinea pig Cellular and Molecular Neuroscience Type IV collagen Laminin Burns Chemical medicine Animals Sodium Hydroxide Corneal epithelium Basement membrane Wound Healing biology Chemistry Cell Differentiation General Medicine Anatomy eye diseases Sensory Systems Eye Burns Ophthalmology medicine.anatomical_structure biology.protein Female Collagen sense organs Conjunctiva Ocular surface |
| Zdroj: | Ophthalmic Research. 27:129-135 |
| ISSN: | 1423-0259 0030-3747 |
| Popis: | To investigate the changes in the corneal epithelial basement membrane following an alkali burn, we examined the immunolocalization of type IV collagen and laminin in the eye of the guinea pig burned with alkali. The burn damaged the corneal, limbal and conjunctival epithelium. After regeneration, basement membrane was interrupted, as indicated by laminin immunoreactivity. Type IV collagen immunoreactivity was transiently expressed in the early healing phase in the epithelial derived from both the cornea and conjunctiva, but was not seen in the normal corneal epithelial basement membrane. Later in the healing process, following transdifferentiation of the conjunctival epithelium into a cornea-like epithelium, its type IV collagen immunoreactivity was weaker than that in the basement membrane of the nontransdifferentiated epithelium. Conjunctival transdifferentiation during healing may have led to transient development of type IV collagen immunoreactivity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|