Overlapping expression and redundant activation of mesenchymal fibroblast growth factor (FGF) receptors by alternatively spliced FGF-8 ligands
| Autor: | Marianne L. Seto, Allison G. Blunt, Avril Lawshé, Craig A. MacArthur, David M. Ornitz, Michael L. Cunningham |
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| Rok vydání: | 1997 |
| Předmět: |
Gene isoform
Fibroblast Growth Factor 8 Alternative splicing Chromosome Mapping Cell Biology Exons Biology Fibroblast growth factor Biochemistry Embryonic stem cell Molecular biology Receptors Fibroblast Growth Factor Neoplasm Proteins Fibroblast Growth Factors Paracrine signalling Alternative Splicing Mice FGF8 Fibroblast growth factor receptor Animals Electrophoresis Polyacrylamide Gel Receptor Growth Substances Molecular Biology |
| Zdroj: | The Journal of biological chemistry. 272(6) |
| ISSN: | 0021-9258 |
| Popis: | FGF-8 is a member of the family of fibroblast growth factors and is expressed during vertebrate embryo development. Eight potential FGF-8 isoforms are generated by alternative splicing in mice, several of which are expressed during embryogenesis in epithelial locations. The significance of the multiple isoforms is currently unknown. In this report, we investigate the expression patterns and the specificity of the FGF-8 isoforms for known fibroblast growth factor (FGF) receptors. RNAs for seven of the eight potential isoforms are present at multiple sites of embryonic Fgf8 expression. None of the FGF-8 isoforms exhibited activity when assayed with BaF3 cells expressing the "b" splice forms of FGF receptors 1-3, which are mostly expressed in epithelial tissues. Mesenchymally expressed "c" splice forms of FGF receptors 2 and 3 and FGF receptor 4 were activated by several FGF-8 isoforms. These findings are consistent with the hypothesis that the multiple FGF-8 isoforms are functionally redundant and function to signal in paracrine (epithelial to mesenchymal) contexts. |
| Databáze: | OpenAIRE |
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