A panel of embryonic stem cell lines reveals the variety and dynamic of pluripotent states in rabbits

Autor: Maxime Aubry, Catherine Archilla, Hervé Acloque, Alain Roulet, Cécile Donnadieu, Yann Tapponnier, Thierry Joly, Pierre Osteil, Pierre Savatier, Luc Jouneau, Anaïs Moulin, Véronique Duranthon, Harmonie Barasc, Barbara Schmaltz-Panneau, Jérôme Lecardonnel, Nathalie Mouney-Bonnet, Marielle Afanassieff, Clémence Genthon, Elen Gócza, Claire Santamaria
Přispěvatelé: ProdInra, Migration, Institut cellule souche et cerveau (U846 Inserm - UCBL1), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM), Children's Medical Research Institute [Westmead, Australia], Institut supérieur d'agriculture et d'agroalimentaire Rhône-Alpes (I.S.A.R.A.), AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Biologie du Développement et Reproduction (BDR), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT], Agricultural Biotechnology Institute, ANR projects PLURABBIT no. PCS-09- GENM-08 and ORYCTOGENE no. ANR-12-RPIB-0013, European Cooperation in Science and Technology (COST) Action (Rabbit Genome Biology [RGB]-Net no. TD1101), HyPharm, Région Rhône-Alpes (ADR CIBLE 2010 project no. R10065CC to Y.T.), Infrastructure Nationale en Biologie et Santé INGESTEM (ANR- 11-INBS-0009), Infrastructure Nationale en Biologie et Santé CRB-Anim (ANR-11-INBS-0003), IHU-B CESAME (ANR-10-IHUB- 003), LabEx DEVweCAN (ANR-10-LABX-0061), LabEx CORTEX (ANR-11-LABX-0042) of the University of Lyon within the program Investissements d’Avenir (ANR-11-IDEX-0007), Fondation pour la Recherche Médicale (FRM SPE20140129375), Institut supérieur d'agriculture et d'agroalimentaire Rhône-Alpes (ISARA), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), AgroParisTech-Institut National de la Recherche Agronomique (INRA), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Cell Culture Techniques
derivation
Embryoid body
Fibroblast growth factor
Biochemistry
Leukemia Inhibitory Factor
generation
Inner cell mass
Cell Self Renewal
Induced pluripotent stem cell
lcsh:QH301-705.5
[SDV.BDD]Life Sciences [q-bio]/Development Biology
Cells
Cultured
reproductive and urinary physiology
Genetics
lcsh:R5-920
Cell Cycle
Biologie du développement
Gene Expression Regulation
Developmental
Embryo
Cell Differentiation
Development Biology
Cell biology
embryonic structures
Rabbits
biological phenomena
cell phenomena
and immunity
lcsh:Medicine (General)
Signal Transduction
cellule souche embryonnaire
rabbit
ESC
Biology
Article
Cell Line
03 medical and health sciences
[SDV.BDD] Life Sciences [q-bio]/Development Biology
pluripotence
Animals
lapin
Embryonic Stem Cells
Janus Kinases
urogenital system
Gene Expression Profiling
Computational Biology
Cell Biology
Embryonic stem cell
MicroRNAs
030104 developmental biology
Blastocyst
lcsh:Biology (General)
Epiblast
Cell culture
Transcriptome
Biomarkers
Developmental Biology
Zdroj: Stem Cell Reports
Stem Cell Reports, 2016, 7 (3), pp.383-398. ⟨10.1016/j.stemcr.2016.07.022⟩
Stem Cell Reports, Elsevier, 2016, 7 (3), pp.383-398. ⟨10.1016/j.stemcr.2016.07.022⟩
Stem Cell Reports 3 (7), 383-398. (2016)
Stem Cell Reports, Vol 7, Iss 3, Pp 383-398 (2016)
ISSN: 2213-6711
Popis: Summary Conventional rabbit embryonic stem cell (ESC) lines are derived from the inner cell mass (ICM) of pre-implantation embryos using methods and culture conditions that are established for primate ESCs. In this study, we explored the capacity of the rabbit ICM to give rise to ESC lines using conditions similar to those utilized to generate naive ESCs in mice. On single-cell dissociation and culture in fibroblast growth factor 2 (FGF2)-free, serum-supplemented medium, rabbit ICMs gave rise to ESC lines lacking the DNA-damage checkpoint in the G1 phase like mouse ESCs, and with a pluripotency gene expression profile closer to the rabbit ICM/epiblast profiles. These cell lines can be converted to FGF2-dependent ESCs after culture in conventional conditions. They can also colonize the rabbit pre-implantation embryo. These results indicate that rabbit epiblast cells can be coaxed toward different types of pluripotent stem cells and reveal the dynamics of pluripotent states in rabbit ESCs.
Highlights • Generation of rabbit ESCs lacking DNA-damage checkpoint in G1 • Transcriptional profiles of rabbit ESC lines vary depending on culture conditions • Rabbit ESCs can switch between states, revealing plasticity • Some rabbit ESCs can colonize the rabbit pre-implantation embryo
Savatier, Afanassieff, and colleagues report the generation of rabbit ESC lines with gene expression profiles and cell-cycle characteristics closer to those of the rabbit epiblast and mouse ESCs than what was previously reported for rbESC conventional cells. These ESC lines can switch between different states, revealing the dynamics of pluripotency states. Some of these ESC lines acquired the capacity to colonize the rabbit embryo.
Databáze: OpenAIRE
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