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Additional file 1: Fig. S1 Flow chart of breeding by molecular design; Fig. S2 Distribution of the target genes in selected parent lines. Black represents yield-related genes, pink represents eating and cooking quality related genes, red represents grain shape related genes, blue represents rice BPH resistance related genes. Uppercase letters represent functional alleles and lowercase letters represent nonfunctional alleles. (Gene description in Table S8); Fig. S3 BPH resistance score of the parents and BC3F4 lines at the seedling stage. 9311 used as susceptible control, LY69 used as resistant control. Values are the means �� s.d., n = 30. Letters indicate a significant difference at the 5% significance level by the least significant difference test; Fig. S4 Genotyping of the target genes in BC3F4 lines. Black represents yield-related genes, pink represents eating and cooking quality related genes, red represents grain shape related genes, blue represents rice BPH resistance related genes. Uppercase letters represent functional alleles and lowercase letters represent nonfunctional alleles. Lines 07, 08 and 14 were subsequently selected and renamed MD1, MD2 and MD3, respectively. (Gene description in Table S8.); Fig. S5 Gross plant and panicle morphologies of the BC3F4 lines and their parents. Scale bars, 10 cm; Fig. S6 Genotypes of the 18 selected lines based on the 15 target genes. Black represents yield-related genes, pink represents eating and cooking quality related genes, red represents grain shape related genes, blue represents rice BPH resistance related genes. Uppercase letters represent functional alleles and lowercase letters represent nonfunctional alleles. (Phenotypic data in Tables S5 and S6. Gene description in Table S8); Fig. S7 Yield-related traits in groups with different target gene combinations. a: Plant height (cm); b: Panicle number; c: Spikelet fertility (%); d: 1000-grian weight (g). Values are the means �� s.d.. Letters indicate a significant difference at the 5% significance level by the least significant difference test; Fig. S8 BPH resistance test of the designed breeding lines at the seedling stage. Values are the means �� s.d., n = 30. Letters indicate a significant difference at the 5% significance level by the least significant difference test; Fig. S9 Target genes and genetic background analysis of the molecular designed breeding lines. Letters A, B and C represent the molecular design breeding lines MD1, MD2 and MD3, respectively. The box color in parenthesis indicates the substituted chromosome segments of the donor parents 1880 (black), Luo-Yu-Xiang, LYX (green), Luoyang-6, LY6 (red) and Luoyang-9, LY9 (blue) with 9311 as the recurrent background (white). |
