Escargot Restricts Niche Cell to Stem Cell Conversion in the Drosophila Testis
| Autor: | Margaret T. Fuller, Luís Pedro F. Resende, Tony Hunter, Gary R. Hime, Justin C. Voog, John R. Yates, D. Leanne Jones, Mariano A. Loza-Coll, Aaron Aslanian, Sharsti Sandall |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Somatic cell Cell Cell fate determination Biology Article General Biochemistry Genetics and Molecular Biology Germline 03 medical and health sciences 0302 clinical medicine Testis medicine Animals Drosophila Proteins Cell Lineage Stem Cell Niche lcsh:QH301-705.5 Transcription factor Alleles 030304 developmental biology Genetics 0303 health sciences Stem Cells Cell biology Germ Cells medicine.anatomical_structure lcsh:Biology (General) Drosophila Stem cell Corepressor 030217 neurology & neurosurgery Drosophila Protein Transcription Factors |
| Zdroj: | Cell Reports, Vol 7, Iss 3, Pp 722-734 (2014) |
| ISSN: | 2211-1247 |
| Popis: | SummaryStem cells reside within specialized microenvironments, or niches, that control many aspects of stem cell behavior. Somatic hub cells in the Drosophila testis regulate the behavior of cyst stem cells (CySCs) and germline stem cells (GSCs) and are a primary component of the testis stem cell niche. The shutoff (shof) mutation, characterized by premature loss of GSCs and CySCs, was mapped to a locus encoding the evolutionarily conserved transcription factor Escargot (Esg). Hub cells depleted of Esg acquire CySC characteristics and differentiate as cyst cells, resulting in complete loss of hub cells and eventually CySCs and GSCs, similar to the shof mutant phenotype. We identified Esg-interacting proteins and demonstrate an interaction between Esg and the corepressor C-terminal binding protein (CtBP), which was also required for maintenance of hub cell fate. Our results indicate that niche cells can acquire stem cell properties upon removal of a single transcription factor in vivo. |
| Databáze: | OpenAIRE |
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