Caffeine inhibits hepatitis C virus replication in vitro
| Autor: | Paula Rahal, Mariana Nogueira Batista, Ana Claudia Silva Braga, Bruno Moreira Carneiro |
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| Rok vydání: | 2013 |
| Předmět: |
Liver Cirrhosis
Cirrhosis Cell Survival Hepatitis C virus Hepacivirus Apoptosis Pharmacology medicine.disease_cause Virus Replication Antiviral Agents Cell Line chemistry.chemical_compound Virology Caffeine medicine Humans biology General Medicine Hepatitis C Cell Cycle Checkpoints medicine.disease biology.organism_classification digestive system diseases chemistry Viral replication Liver Hepatocellular carcinoma Hepatocytes |
| Zdroj: | Archives of virology. 160(2) |
| ISSN: | 1432-8798 |
| Popis: | Hepatitis C is considered the major cause of cirrhosis and hepatocellular carcinoma. Conventional treatment is not effective against some hepatitis C virus (HCV) genotypes; therefore, new treatments are needed. Coffee and, more recently, caffeine, have been found to have a beneficial effect in several disorders of the liver, including those manifesting abnormal liver biochemistry, cirrhosis and hepatocellular carcinoma. Caffeine acts directly by delaying fibrosis, thereby improving the function of liver cellular pathways and interfering with pathways used by the HCV replication cycle. In the current study, the direct relationship between caffeine and viral replication was evaluated. The Huh-7.5 cell line was used for transient infections with FL-J6/JFH-5′C19Rluc2AUbi and to establish a cell line stably expressing SGR-Feo JFH-1. Caffeine efficiently inhibited HCV replication in a dose-dependent manner at non-cytotoxic concentrations and demonstrated an IC50 value of 0.7263 mM after 48 h of incubation. These data demonstrate that caffeine may be an important new agent for anti-HCV therapies due to its efficient inhibition of HCV replication at non-toxic concentrations. |
| Databáze: | OpenAIRE |
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