Steroidogenic acute regulatory protein expression is dependent upon post-translational effects of cAMP-dependent protein kinase A
| Autor: | Barbara J. Clark, Velvizhi Ranganathan, Rebecca Combs |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
endocrine system
Blotting Western Molecular Sequence Data 8-Bromo Cyclic Adenosine Monophosphate Biology Biochemistry Cell Line Mice Endocrinology Polysome Animals Cholesterol Side-Chain Cleavage Enzyme RNA Messenger Phosphorylation Protein kinase A Molecular Biology Messenger RNA urogenital system Steroidogenic acute regulatory protein Translation (biology) Isoquinolines Phosphoproteins Cyclic AMP-Dependent Protein Kinases Precipitin Tests Molecular biology Cell biology Gene Expression Regulation Cell culture Signal transduction Protein Processing Post-Translational |
| Zdroj: | Molecular and Cellular Endocrinology. 173:183-192 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/s0303-7207(00)00410-x |
| Popis: | Tropic hormones acutely stimulate adrenal and gonadal steroidogenesis by activation of the cAMP-dependent protein kinase A (PKA) signaling pathway and subsequent induction of Steroidogenic Acute Regulatory (StAR) protein (StAR) expression. We present a comparative study of StAR regulation in mouse adrenocortical Y1 and the derived PKA mutant Kin-8 cell lines to evaluate the PKA requirement for StAR expression. A parallel increase in StAR steady-state mRNA and protein was observed in Y1 cells. StAR mRNA was induced in 8-Br-cAMP-treated Kin-8 cells with maximal expression levels approx. 50% of that observed in Y1 cells. However, a corresponding increase in StAR protein, as detected by Western analysis, was absent in the Kin-8 cells. A similar distribution of StAR mRNA in active polysome fractions was observed for both 8-Br-cAMP-treated Y1 and Kin-8 cells, as well as a 2-fold increase in incorporation of [35S]methionine into StAR, which indicated translation was not blocked in Kin-8 cells. Together these data indicate that PKA functions at the post-translational level to regulate StAR expression and we propose that phosphorylation of StAR by PKA contributes to protein stability |
| Databáze: | OpenAIRE |
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