The homoeologous genes encoding chalcone-flavanone isomerase in Triticum aestivum L.: structural characterization and expression in different parts of wheat plant

Autor: Elena K. Khlestkina, Hélène Bergès, Olesya Yu. Shoeva, Elena A. Salina
Přispěvatelé: Inst Cytol & Genet, Siberian Branch, the Russian Academy of Sciences [Moscow, Russia] (RAS), Novosibirsk State University (NSU), Inst Cytol & Genet, Russian Academy of Sciences [Moscow] (RAS), Centre National de Ressources Génomiques Végétales (CNRGV), Institut National de la Recherche Agronomique (INRA), RFBR [12-04-33027], Russian Federation [MD-2615.2013.4], State Budget Programme [VI.53.1.5.]
Rok vydání: 2013
Předmět:
0106 biological sciences
FLAVONOBIOSYNTHESIS
Protein Conformation
[SDV]Life Sciences [q-bio]
Gene Expression
SYNTHASE PROMOTER
01 natural sciences
Genome
Transcription (biology)
Gene expression
Tissue Distribution
Cloning
Molecular
Intramolecular Lyases
Promoter Regions
Genetic
Phylogeny
Triticum
Regulator gene
Plant Proteins
2. Zero hunger
Genetics
0303 health sciences
Intron-exon structure
food and beverages
General Medicine
Exons
GENOME
TRANSCRIPTION FACTORS
Chalcone-flavanone isomerase
Ploidy
DUPLICATED GENES
SEQUENCE TAGS
Flavonoid biosynthesis
Triticum aestivum L
ANTHOCYANIN PIGMENTATION
DNA
Plant
Molecular Sequence Data
Biology
Genes
Plant
Chromosomes
Plant
Evolution
Molecular
Polyploidy
03 medical and health sciences
Species Specificity
Sequence Homology
Nucleic Acid
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Amino Acid Sequence
Gene
030304 developmental biology
COMMON WHEAT
Base Sequence
Sequence Homology
Amino Acid
Intron
EVOLUTION
Introns
Modems
Homoeologous genes
HEXAPLOWHEAT
010606 plant biology & botany
Zdroj: Gene
Gene, Elsevier, 2014, 538 (2), pp.334-341. ⟨10.1016/j.gene.2014.01.008⟩
ISSN: 1879-0038
0378-1119
DOI: 10.1016/j.gene.2014.01.008⟩
Popis: International audience; Chalcone-flavanone isomerase (CHI; EC 5.5.1.6.) participates in the early step of flavonoid biosynthesis, related to plant adaptive and protective responses to environmental stress. The bread wheat genomic sequences encoding CHI were isolated, sequenced and mapped to the terminal segment of the long arms of chromosomes 5A, 5B and 5D. The loss of the final Chi intron and junction of the two last exons was found in the wheat A, B and D genomes compared to the Chi sequences of most other plant species. Each of the three diploid genomes of hexaploid wheat encodes functional CHI; however, transcription of the three homoeologous genes is not always co-regulated. In particular, the three genes demonstrated different response to salinity in roots: Chi-D1 was upregulated, Chi-A1 responds medially, whereas Chi-B1 was not activated at all. The observed variation in transcriptional activity between the Chi homoeologs is in a good agreement with structural diversification of their promoter sequences. In addition, the correlation between Chi transcription and anthocyanin pigmentation in different parts of wheat plant has been studied. The regulatory genes controlling anthocyanin pigmentation of culm and pericarp modulated transcription of the Chi genes. However, in other organs, there was no strong relation between tissue pigmentation and the transcription of the Chi genes, suggesting complex regulation of the Chi expression in most parts of wheat plant (C) 2014 Elsevier B.V. All rights reserved.
Databáze: OpenAIRE
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