Polarization response of second-harmonic images for different collagen spatial distributions
| Autor: | Francisco J. Ávila, Oscar del Barco, Juan M. Bueno |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Diagnostic Imaging Materials science Optical fiber Biomedical Engineering Polarimetry 01 natural sciences law.invention 010309 optics Biomaterials 03 medical and health sciences Optics law 0103 physical sciences Microscopy Humans Statistical analysis business.industry Collagen Diseases Second-harmonic generation Polarization (waves) Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials 030104 developmental biology Clinical diagnosis Collagen Microscopy Polarization business Internal organization |
| Zdroj: | Journal of biomedical optics. 21(6) |
| ISSN: | 1560-2281 |
| Popis: | The response to polarization of second-harmonic generation (SHG) microscopy images of samples with different collagen distributions (quasialigned, partially organized, and nonorganized) has been analyzed. A linear decay relationship between the external arrangement and polarization sensitivity was found. SHG signal from nonorganized samples presented a large structural dispersion and a weak dependence with incident polarization. Polarization dependence is also associated with the internal organization of the collagen fibers, directly related to the ratio of hyperpolarizabilities ρ. This parameter can experimentally be computed from the modulation of the SHG signal. The results show that both external and internal collagen structures are closely related. This provides a tool to obtain information of internal properties from the polarimetric response of the external spatial distribution of collagen, which might be useful in clinical diagnosis of pathologies related to changes in collagen structure. |
| Databáze: | OpenAIRE |
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