Label-free Rapid Viable Enrichment of Circulating Tumor Cell by Photosensitive Polymer-based Microfilter Device

Autor: Young-Ho Cho, Jiyoung Byun, Yoon-Tae Kang, Hee Jin Chang, Il Doh
Rok vydání: 2017
Předmět:
Light
Cell Survival
Polymers
viable rare cell isolation
Fluorescent Antibody Technique
Medicine (miscellaneous)
02 engineering and technology
Models
Biological
01 natural sciences
Circulating tumor cell
Cell Line
Tumor
Neoplasms
medicine
Humans
Cell Shape
Pharmacology
Toxicology and Pharmaceutics (miscellaneous)
MUC1
Label free
photosensitive polymer
Staining and Labeling
Chemistry
010401 analytical chemistry
Circulating tumor cells
Reproducibility of Results
Cancer
clinical cancer study
Cell concentration
Neoplastic Cells
Circulating
021001 nanoscience & nanotechnology
medicine.disease
Molecular biology
0104 chemical sciences
Gene Expression Regulation
Neoplastic
tapered-slit filter
Cancer cell
Cancer research
Microtechnology
0210 nano-technology
Photosensitive polymer
Filtration
Research Paper
Cell based
Zdroj: Theranostics
ISSN: 1838-7640
DOI: 10.7150/thno.19686
Popis: We present a clinical device for simple, rapid, and viable isolation of circulating tumor cells (CTCs) from cancer patient bloods. In spite of the clinical importance of CTCs, the lack of easy and non-biased isolation methods is a big hurdle for implementing CTC into clinical use. The present device made of photosensitive polymer was designed to attach to conventional syringe to isolate the CTCs at minimal resources. Its unique tapered-slits on the filter are capable not only to isolate the cell based on their size and deformability, but also to increase sample flow rate, thus achieving label-free rapid viable CTC isolation. We verified our device performance using 9 different types of cancer cells at the cell concentration from 5 to 100cells/ml, showing that the device capture 77.7% of the CTCs while maintaining their viability of 80.6%. We extended our study using the 18 blood samples from lung, colorectal, pancreatic and renal cancer patients and captured 1-172 CTCs or clustered CTCs by immunofluorescent or immunohistochemical staining. The captured CTCs were also molecularly assayed by RT-PCR with three cancer-associated genes (CK19, EpCAM, and MUC1). Those comprehensive studies proved to use our device for cancer study, thereby inaugurating further in-depth CTC-based clinical researches.
Databáze: OpenAIRE
Externí odkaz: