Inhibition of rotavirus infection in vitro and in vivo by a synthetic peptide from VP4
| Autor: | S. Uduman, D. Dent, S.K. Attah-Poku, M.K. Ijaz, L. A. Babiuk, M.S.A. Nur-E-Kamal, M.J. Redmond, F.K. Dar |
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| Rok vydání: | 1998 |
| Předmět: |
Rotavirus
viruses Molecular Sequence Data Reoviridae Biology medicine.disease_cause Rotavirus Infections Virus Epitope Cell Line Microbiology Mice Capsid fluids and secretions In vivo medicine Animals Trypsin Amino Acid Sequence Peptide sequence Vaccines Synthetic Binding Sites General Veterinary General Immunology and Microbiology Public Health Environmental and Occupational Health virus diseases Viral Vaccines biology.organism_classification Virology Rotavirus vaccine Peptide Fragments In vitro Infectious Diseases Receptors Virus Molecular Medicine Capsid Proteins Cattle |
| Zdroj: | Vaccine. 16:916-920 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/s0264-410x(97)00298-3 |
| Popis: | A synthetic peptide corresponding to bovine rotavirus C486 (BRV) VP4 amino acid sequence 232–255 (VP4-peptide) was studied with the objective of defining the origin of the protective immune response reported previously by Ijaz et al. (J. Virol. 1991, 65 , 3106–3113). Pretreatment of MA-104 cells with the VP4-peptide before infection with rotavirus prevented both the attachment of 35 S-labelled virus and plaque formation in vitro. In vivo studies using a murine rotavirus model demonstrated that intragastric administration of VP4-peptide protected subjects from challenge with virulent rotavirus. These results clearly indicate the importance of this epitope in virus-cell interactions and their potential as a rotavirus vaccine candidate. |
| Databáze: | OpenAIRE |
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