A defined range of guard cell calcium oscillation parameters encodes stomatal movements
| Autor: | Carrie L. Harrington, Karin Schumacher, Yat Y. Tang, Sarah P. Chu, Erwin Grill, Julian I. Schroeder, Thomas Hoffmann, Gethyn J. Allen |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Arabidopsis
chemistry.chemical_element In Vitro Techniques Calcium environment and public health chemistry.chemical_compound Guard cell Extracellular Arabidopsis thaliana Calcium Signaling Abscisic acid Calcium metabolism Multidisciplinary biology Chemistry Wild type biology.organism_classification Electrophysiology Plant Leaves enzymes and coenzymes (carbohydrates) Cytosol Biochemistry Mutation embryonic structures cardiovascular system Biophysics Abscisic Acid |
| Zdroj: | Nature. 411:1053-1057 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/35082575 |
| Popis: | Oscillations in cytosolic calcium concentration ([Ca2+]cyt) are central regulators of signal transduction cascades, although the roles of individual [Ca2+]cyt oscillation parameters in regulating downstream physiological responses remain largely unknown. In plants, guard cells integrate environmental and endogenous signals to regulate the aperture of stomatal pores and [Ca2+]cyt oscillations are a fundamental component of stomatal closure. Here we systematically vary [Ca2+]cyt oscillation parameters in Arabidopsis guard cells using a 'calcium clamp' and show that [Ca2+]cyt controls stomatal closure by two mechanisms. Short-term 'calcium-reactive' closure occurred rapidly when [Ca2+]cyt was elevated, whereas the degree of long-term steady-state closure was 'calcium programmed' by [Ca2+]cyt oscillations within a defined range of frequency, transient number, duration and amplitude. Furthermore, in guard cells of the gca2 mutant, [Ca2+]cyt oscillations induced by abscisic acid and extracellular calcium had increased frequencies and reduced transient duration, and steady-state stomatal closure was abolished. Experimentally imposing [Ca2+]cyt oscillations with parameters that elicited closure in the wild type restored long-term closure in gca2 stomata. These data show that a defined window of guard cell [Ca2+]cyt oscillation parameters programs changes in steady-state stomatal aperture. |
| Databáze: | OpenAIRE |
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