Low power microwave interaction with phospholipase C and D signal transduction pathways in myogenic cells
| Autor: | Paola Ceci, Andrea Donato, Francesco Tomei, Fabio Naro, Andrea Cannavò |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Phospholipase
Biology Cell Line Myoblasts avp medicine Phospholipase D Animals phospholipase Microwaves Phospholipase C Myogenesis acetylcholine mw myogenic cells Dose-Response Relationship Radiation Cell Biology General Medicine Rats Nicotinic acetylcholine receptor Biochemistry Type C Phospholipases Biophysics Signal transduction Intracellular Acetylcholine medicine.drug Signal Transduction |
| Zdroj: | Cell biology international. 28(10) |
| ISSN: | 1065-6995 |
| Popis: | Ionic channel proteins are possible sites of microwave interaction at the cell membrane level. Patch-clamp data, using single channel and total current recording, indicated that low level microwave fields may modify some functional parameters of the nicotinic acetylcholine receptor in primary chick myotubes, suggesting a possible effect of microwaves on myogenic cells. Here, we investigated the biological relevance of such results, in relation to the possible involvement of intracellular signaling processes. We exposed L6–C5 myogenic cells to low power electromagnetic fields and observed the consequences on hormonal activation of phospholipases C and D. We found that increased inositol phospholipid turnover, induced by acetylcholine and arginine vasopressin activation of phospholipase C, was not modified in microwave irradiated myoblasts or myotubes. Moreover, vasopressin-dependent phospholipase D activation, assessed by measuring the [3H]-free choline release, was not modified by microwave irradiation. Our conclusions suggest that low level microwave fields do not modify signal transduction pathways activated by acetylcholine and vasopressin in L6–C5 myogenic cells. |
| Databáze: | OpenAIRE |
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