Up-regulation of 15-lipoxygenase enzymes and products in functional and non-functional pituitary adenomas

Autor: Alimohammad Alimohammadi, Masoumeh Tavakoli-Yaraki, Mitra Nourbakhsh, Alireza Sheikhi, Hamideh Akbari, Alaleh Bayat Barooni, Mehrnaz Imani, Vahid Salimi, Maryam Ameri, Farzad Izak Shirian, Mohammad E. Khamseh, Mohammad Ghorbani
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Gene isoform
Adenoma
Adult
Male
endocrine system diseases
Clinical chemistry
Endocrinology
Diabetes and Metabolism
Clinical Biochemistry
030209 endocrinology & metabolism
030204 cardiovascular system & hematology
Gene Expression Regulation
Enzymologic
Pathogenesis
03 medical and health sciences
Young Adult
0302 clinical medicine
Endocrinology
Downregulation and upregulation
Pituitary adenoma
Hydroxyeicosatetraenoic Acids
medicine
Arachidonate 15-Lipoxygenase
Humans
Pituitary Neoplasms
lcsh:RC620-627
Aged
chemistry.chemical_classification
business.industry
Research
Biochemistry (medical)
Pituitary tumors
13(S) HODE
Middle Aged
medicine.disease
Up-Regulation
Gene Expression Regulation
Neoplastic
lcsh:Nutritional diseases. Deficiency diseases
Enzyme
chemistry
Linoleic Acids
Case-Control Studies
Cancer research
15(S) HETE
Female
business
15-Lipoxygense
Lipidology
Zdroj: Lipids in Health and Disease
Lipids in Health and Disease, Vol 18, Iss 1, Pp 1-11 (2019)
ISSN: 1476-511X
Popis: Background Pituitary adenoma accounts as a complex and multifactorial intracranial neoplasm with wide range of clinical symptoms which its underlying molecular mechanism has yet to be determined. The bioactive lipid mediators received attentions toward their contribution in cancer cell proliferation, progression and death. Amongst, 15-Lipoxygense (15-Lox) enzymes and products display appealing role in cancer pathogenesis which their possible effect in pituitary adenoma tumor genesis is perused in the current study. Methods The 15-Lipoxygenses isoforms expression level was evaluated in tumor tissues of prevalent functional and non-functional pituitary adenomas and normal pituitary tissues via Real-Time PCR. The circulating levels of 15(S) HETE and 13(S) HODE as 15-Lox main products were assessed in serum of patients and healthy subjects using enzyme immunoassay kits. Results Our results revealed that 15-Lox-1 and 15-Lox-2 expression levels were elevated in tumor tissues of pituitary adenomas comparing to normal pituitary tissues. The elevated levels of both isoforms were accompanied with 15(S) HETE and 13(S) HODE elevation in the serum of patients. The 15-Lox-1 expression and activity was higher in invasive tumors as well as tumors with bigger size indicating the possible pro-tumorigenic role of 15-Lox-1, more than 15-Lox-2 in pituitary adenomas. The diagnostic value of 15-Lipoxygense isoforms and products were considerable between patients and healthy groups. Conclusion The possible involvement of 15-Lipoxygense pathway especially 15-Lox-1 in the regulation of pituitary tumor growth and progression may open up new molecular mechanism regarding pituitary adenoma pathogenesis and might shed light on its new therapeutic strategies.
Databáze: OpenAIRE
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