Evaluation of Fucosylated Haptoglobin and Mac-2 Binding Protein as Serum Biomarkers to Estimate Liver Fibrosis in Patients with Chronic Hepatitis C
| Autor: | Tomohide Tatsumi, Sadaharu Iio, Ichizou Kobayashi, Tetsuo Takehara, Seiichi Tawara, Ryotaro Sakamori, Minoru Shigekawa, Naoki Hiramatsu, Eiji Miyoshi, Hayato Hikita |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Liver Cirrhosis
Male RNA viruses Chronic Hepatitis Pathology Biopsy lcsh:Medicine Hepacivirus medicine.disease_cause Gastroenterology Chronic Liver Disease 0302 clinical medicine Fibrosis Nonalcoholic fatty liver disease Medicine and Health Sciences Medicine Enzyme-Linked Immunoassays lcsh:Science Pathology and laboratory medicine Membrane Glycoproteins Multidisciplinary biology Hepatitis C virus Liver Diseases Liver Neoplasms Fatty liver Haptoglobin Hepatitis C Middle Aged Medical microbiology Oncology 030220 oncology & carcinogenesis Hepatocellular carcinoma Viruses Liver Fibrosis Female 030211 gastroenterology & hepatology Pathogens Research Article medicine.medical_specialty Carcinoma Hepatocellular Enzyme-Linked Immunosorbent Assay Surgical and Invasive Medical Procedures Gastroenterology and Hepatology Research and Analysis Methods Carcinomas Microbiology 03 medical and health sciences Antigens Neoplasm Internal medicine Gastrointestinal Tumors Humans Immunoassays Retrospective Studies Haptoglobins Flaviviruses business.industry lcsh:R Organisms Viral pathogens Biology and Life Sciences Cancers and Neoplasms Hepatocellular Carcinoma Hepatitis C Chronic medicine.disease Hepatitis viruses Microbial pathogens Fatty Liver Immunologic Techniques biology.protein lcsh:Q business Hepatic fibrosis Biomarkers Developmental Biology |
| Zdroj: | PLoS ONE, Vol 11, Iss 3, p e0151828 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Fucosylated haptoglobin (Fuc-Hpt) and Mac-2 binding protein (Mac-2 bp) are identified as cancer biomarkers, based on the results from a glyco-proteomic analysis. Recently, we reported that these glyco-biomarkers were associated with liver fibrosis and/or ballooning hepatocytes in patients with nonalcoholic fatty liver disease (NAFLD). We evaluated the ability of these glycoproteins to estimate liver fibrosis in 317 patients with chronic hepatitis C. We measured the serum Fuc-Hpt and Mac-2 bp levels using a lectin-antibody ELISA and ELISA, respectively. The serum levels of both Fuc-Hpt and Mac-2 bp increased with the progression of liver fibrosis. The multivariate analysis revealed that Mac-2 bp was an independent factor associated with moderate liver fibrosis (F ≥ 2). In contrast, Fuc-Hpt was an independent factor associated with advanced liver fibrosis (F ≥ 3). In terms of evaluating liver fibrosis, the serum levels of these glycomarkers were correlated with well-known liver fibrosis indexes, such as the aspartate aminotransferase to platelet ratio index (APRI) and Fibrosis-4 (FIB4) index. An assay that combined the APRI or FIB4 index and the Fuc-Hpt or Mac-2 bp levels increased the AUC value for diagnosing hepatic fibrosis. Interestingly, the cumulative incidence of hepatocellular carcinoma (HCC) was significantly higher in the patients with elevated serum levels of Fuc-Hpt and Mac-2 bp. In conclusion, both Fuc-Hpt and Mac-2 bp could be useful glyco-biomarkers of liver fibrosis and predictors of HCC in patients with chronic hepatitis C. |
| Databáze: | OpenAIRE |
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