Use of a monoclonal antibody in a dot immunobinding assay for detection of a circulating mannoprotein of Candida spp. in neutropenic patients with invasive candidiasis
| Autor: | F De Bernardis, Daniela Adriani, M. Boccanera, Corrado Girmenia, Pietro Martino, Antonio Cassone |
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| Rok vydání: | 1993 |
| Předmět: |
Microbiology (medical)
Antigens Fungal Neutropenia medicine.drug_class Immunoblotting Monoclonal antibody Candida parapsilosis Sensitivity and Specificity Epitope Microbiology Serology Candida tropicalis Antigen Candida krusei Candida albicans medicine Humans Serologic Tests Membrane Glycoproteins biology Candidiasis Antibodies Monoclonal biology.organism_classification Virology Evaluation Studies as Topic Research Article |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.31.12.3142-3146.1993 |
| Popis: | A dot immunobinding assay for the detection of a circulating mannoprotein (MP) antigen of Candida species in the sera of neutropenic patients in a hematological setting is described. The technique is based on the use of a monoclonal antibody which recognizes an oligomannoside epitope shared by distinct MP of pathogenic Candida species. The sensitivity of the assay for antigen detection in serum was between 2 and 5 ng/ml, and MPs from Candida albicans, Candida tropicalis, Torulopsis glabrata, and Candida parapsilosis, but not Candida krusei, could be detected. A retrospective analysis of sera from patients with proven invasive candidiasis versus sera from controls (Candida-colonized and noncolonized subjects) revealed that the novel assay has sufficient sensitivity, specificity, and predictive values to be of potential diagnostic significance. |
| Databáze: | OpenAIRE |
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