Survival rate of preantral follicles derived from vitrified neonate mouse ovarian tissue by Cryotop and conventional methods
Autor: | M. Rezazadeh Valojerdi, S. S. Mamzoji, H. Guorabi, M. M. Soleimani, P Eftekhari Yazdi, Abdolhossein Shahverdi, M. H. Abnosi, Hussein Eimani |
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Rok vydání: | 2008 |
Předmět: |
Gynecology
Cryopreservation medicine.medical_specialty Test group Cell Survival Ovarian tissue Clinical Biochemistry General Medicine Biology Biochemistry Andrology Follicle Mice medicine.anatomical_structure Organ Culture Techniques Ovarian Follicle Follicular phase Toxicity medicine Molecular Medicine Animals Female Ovarian follicle Survival rate |
Zdroj: | BioFactors (Oxford, England). 31(2) |
ISSN: | 0951-6433 |
Popis: | The aim of this study was to investigate the growth and survival rate of preantral follicles isolated from vitrified ovarian tissue by Cryotop and conventional methods. The ovaries of 14-day-old mice were separated and divided into four groups as following: Cryotop group, vitrified by Cryotop; CV (Conventional; CV) group, vitrified by conventional straw; toxicity test group and control group. After warming the vitrified ovaries, isolated preantral follicles from four groups were cultured for 4 days to compare survival rate and follicular growth between above-mentioned groups. Survival rate (97.3%) in toxicity test group alike the control group (98.7%) were significantly higher (P |
Databáze: | OpenAIRE |
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