Culture Medium and Sex Drive Epigenetic Reprogramming in Preimplantation Bovine Embryos

Autor: Pilar Coy, Fernando Perez-Sanz, Sebastian Canovas, Elena Ivanova, Gavin Kelsey, Meriem Hamdi, Dimitrios Rizos
Přispěvatelé: Fundación Séneca, Ministerio de Ciencia e Innovación (España), Biotechnology and Biological Sciences Research Council (UK), Medical Research Council (UK), Sebastian Canovas, Meriem Hamdi, Fernando Perez-Sanz, Dimitrios Rizos, Gavin Kelsey, Pilar Coy, Sebastian Canovas [0000-0003-4190-6258], Meriem Hamdi [0000-0002-2792-8293], Fernando Perez-Sanz [0000-0002-8143-7794], Dimitrios Rizos [0000-0001-6813-3940], Gavin Kelsey [0000-0002-9762-5634], Pilar Coy [0000-0002-3943-1890], Perez-Sanz, Fernando [0000-0002-8143-7794], Rizos, Dimitrios [0000-0001-6813-3940], Kelsey, Gavin [0000-0002-9762-5634], Coy, Pilar [0000-0002-3943-1890], Apollo - University of Cambridge Repository
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Male
0301 basic medicine
Reproductive technology
Epigenesis
Genetic
0302 clinical medicine
Blood serum
Biology (General)
Spectroscopy
Principal Component Analysis
Sex Characteristics
030219 obstetrics & reproductive medicine
DNA methylation
Epigenetic
Embryo
General Medicine
Methylation
Bovine
Cellular Reprogramming
Computer Science Applications
Chemistry
medicine.anatomical_structure
embryonic structures
Female
Reprogramming
QH301-705.5
embryo
Fertilization in Vitro
Biology
Article
Catalysis
Inorganic Chemistry
Andrology
03 medical and health sciences
Sexual dimorphism
medicine
Animals
Epigenetics
Blastocyst
Preimplantation
Physical and Theoretical Chemistry
QD1-999
Molecular Biology
Organic Chemistry
Molecular Sequence Annotation
Chromosomes
Mammalian
Culture Media
Gene Ontology
Logistic Models
030104 developmental biology
Cattle
CpG Islands
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
International Journal of Molecular Sciences
Volume 22
Issue 12
International Journal of Molecular Sciences, Vol 22, Iss 6426, p 6426 (2021)
Popis: Departamento de Reproducción Animal​ (INIA)
Assisted reproductive technologies impact transcriptome and epigenome of embryos and can result in long-term phenotypic consequences. Whole-genome DNA methylation profiles from individual bovine blastocysts in vivo- and in vitro-derived (using three sources of protein: reproductive fluids, blood serum and bovine serum albumin) were generated. The impact of in vitro culture on DNA methylation was analyzed, and sex-specific methylation differences at blastocyst stage were uncovered. In vivo embryos showed the highest levels of methylation (29.5%), close to those produced in vitro with serum, whilst embryos produced in vitro with reproductive fluids or albumin showed less global methylation (25–25.4%). During repetitive element analysis, the serum group was the most affected. DNA methylation differences between in vivo and in vitro groups were more frequent in the first intron than in CpGi in promoters. Moreover, hierarchical cluster analysis showed that sex produced a stronger bias in the results than embryo origin. For each group, distance between male and female embryos varied, with in vivo blastocyst showing a lesser distance. Between the sexually dimorphic methylated tiles, which were biased to X-chromosome, critical factors for reproduction, developmental process, cell proliferation and DNA methylation machinery were included. These results support the idea that blastocysts show sexually-dimorphic DNA methylation patterns, and the known picture about the blastocyst methylome should be reconsidered
This research was funded by Fundación Seneca-Región de Murcia (Spain), grant number 20040/GERM/16, by the Ministry of Science and Innovation (Spain), grant numbers AGL2015–66341-R, AGL-2015-70140-R, PID2019-111641RB-I00 and by the UK Biotechnology and Biological Sciences Research Council and Medical Research Council.
This article belongs to the Special Issue Mammalian Gametes: Molecular Traits Shaping Their Form and Fate
Databáze: OpenAIRE
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