Light and electron microscopic assessment of progressive atrophy following moderate traumatic brain injury in the rat
| Autor: | Alejandra C. Rodriguez-Paez, J. P. Brunschwig, Helen M. Bramlett |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
Pathology medicine.medical_specialty Wallerian degeneration External capsule Traumatic brain injury Thalamus Pathology and Forensic Medicine White matter Rats Sprague-Dawley Cellular and Molecular Neuroscience Microscopy Electron Transmission Medicine Animals Microglia business.industry Brain Anatomy medicine.disease Axons Rats medicine.anatomical_structure nervous system Cerebral cortex Brain Injuries Nerve Degeneration Neurology (clinical) Atrophy business Infiltration (medical) |
| Zdroj: | Acta neuropathologica. 109(6) |
| ISSN: | 0001-6322 |
| Popis: | The presence of progressive white matter atrophy following traumatic brain injury (TBI) has been reported in humans as well as in animal models. However, a quantitative analysis of progressive alterations in myelinated axons and other cellular responses to trauma has not been conducted. This study examined quantitative differences in myelinated axons from several white and gray matter structures between non-traumatized and traumatized areas at several time points up to 1 year. We hypothesize that axonal numbers decrease over time within the structures analyzed, based on our previous work demonstrating shrinkage of tissue in these vulnerable areas. Intubated, anesthetized male Sprague-Dawley rats were subjected to moderate (1.8–2.2 atm) parasagittal fluid-percussion brain injury, and perfused at various intervals after surgery. Sections from the fimbria, external capsule, thalamus and cerebral cortex from the ipsilateral hemisphere of traumatized and sham-operated animals were prepared and. estimated total numbers of myelinated axons were determined by systematic random sampling. Electron micrographs were obtained for ultrastructural analysis. A significant (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink