Quantitative diagnostic imaging of cancer tissues by using phosphor-integrated dots with ultra-high brightness
| Autor: | Yuka Yoshihara, Mika Watanabe, Yasushi Nakano, Yuichi Ozaki, Hideki Goda, Hisashi Hirakawa, Takanori Ishida, Minoru Miyashita, Noriaki Ohuchi, Yayoi Takahashi-Aoyama, Hiroshi Negishi, Kohsuke Gonda, Shin Usami, Hiroshi Tada, Yoichiro Kakugawa, Kensaku Takanashi, Masaru Takahashi, Hisatake Okada, Takashi Kamei, Ryuichi Yoshida, Akihiko Furuta, Yohei Hamanaka |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology Receptor ErbB-2 Biopsy Gene Expression 3 3'-Diaminobenzidine 0302 clinical medicine Antineoplastic Agents Immunological Trastuzumab Perylene Multidisciplinary medicine.diagnostic_test Middle Aged Fluorescence Immunohistochemistry Neoplasm Proteins 030220 oncology & carcinogenesis Medicine Female medicine.drug Diagnostic Imaging medicine.medical_specialty Science Biotin Breast Neoplasms Imides Antibodies Article 03 medical and health sciences Breast cancer medicine Medical imaging Humans Particle Size Fluorescent Dyes business.industry Chromogenic Rhodamines Cancer medicine.disease 030104 developmental biology Nanoparticles Streptavidin business |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) |
| ISSN: | 2045-2322 |
| Popis: | The quantitative sensitivity and dynamic range of conventional immunohistochemistry (IHC) with 3,3′-diaminobenzidine (IHC-DAB) used in pathological diagnosis in hospitals are poor, because enzyme activity can affect the IHC-DAB chromogenic reaction. Although fluorescent IHC can effectively increase the quantitative sensitivity of conventional IHC, tissue autofluorescence interferes with the sensitivity. Here, we created new fluorescent nanoparticles called phosphor-integrated dots (PIDs). PIDs have 100-fold greater brightness and a more than 300-fold greater dynamic range than those of commercially available fluorescent nanoparticles, quantum dots, whose fluorescence intensity is comparable to tissue autofluorescence. Additionally, a newly developed image-processing method enabled the calculation of the PID particle number in the obtained image. To quantify the sensitivity of IHC using PIDs (IHC-PIDs), the IHC-PIDs method was compared with fluorescence-activated cell sorting (FACS), a method well suited for evaluating total protein amount, and the two values exhibited strong correlation (R = 0.94). We next applied IHC-PIDs to categorize the response to molecular target-based drug therapy in breast cancer patients. The results suggested that the PID particle number estimated by IHC-PIDs of breast cancer tissues obtained from biopsy before chemotherapy can provide a score for predicting the therapeutic effect of the human epidermal growth factor receptor 2-targeted drug trastuzumab. |
| Databáze: | OpenAIRE |
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