A polysaccharide isolated from Cordyceps sinensis, a traditional Chinese medicine, protects PC12 cells against hydrogen peroxide-induced injury
| Autor: | Zong H. Song, Shaoping Li, J.K.H. Cheung, Chunkeung Lo, Kuijun Zhao, Karl Wah Keung Tsim, Zhaoning Ji, Shangquan Zhu, Tina Tingxia Dong |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Cell Survival
Mannose Polysaccharide Protective Agents PC12 Cells General Biochemistry Genetics and Molecular Biology Superoxide dismutase chemistry.chemical_compound Immune system Polysaccharides Malondialdehyde Animals General Pharmacology Toxicology and Pharmaceutics Medicine Chinese Traditional Hydrogen peroxide chemistry.chemical_classification Cordyceps Glutathione Peroxidase biology Superoxide Dismutase Glutathione peroxidase Electrophoresis Capillary General Medicine Hydrogen Peroxide biology.organism_classification Rats chemistry Biochemistry Galactose biology.protein |
| Zdroj: | Life sciences. 73(19) |
| ISSN: | 0024-3205 |
| Popis: | Cordyceps sinensis, a well-known traditional Chinese medicine, possesses activities in anti-tumour, anti-oxidation and stimulating the immune system; however, the identity of active component(s) is not determined. By using anti-oxidation activity-guided fractionation, a polysaccharide of molecular weight approximately 210 kDa was isolated from cultured Cordyceps mycelia by ion-exchange and sizing chromatography. The isolated polysaccharide, having strong anti-oxidation activity, contains glucose, mannose and galactose in a ratio of 1 : 0.6 : 0.75. The pre-treatment of isolated polysaccharide on the cultured rat pheochromocytoma PC12 cells shows strong protective effect against hydrogen peroxide (H(2)O(2))-induced insult. Treatment of the cells with the isolated polysaccharide at 100 microg/ml prior to H(2)O(2) exposure significantly elevated the survival of PC12 cells in culture by over 60%. In parallel, the H(2)O(2)-induced production of malondialdehyde in cultured cells was markedly reduced by the polysaccharide treatment. Moreover, the pre-treatment of the isolated polysaccharide significantly attenuated the changes of glutathione peroxidase and superoxide dismutase activities in H(2)O(2)-treated cells in a dose-dependent manner. This is the first report in identifying a polysaccharide from Cordyceps, which protects against the free radical-induced neuronal cell toxicity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect