Impact of a 1.5 T magnetic field on DNA damage in MRI-guided HDR brachytherapy
| Autor: | Loni Fuller, Erika L. Brescia, Mauro Tambasco, Geordi Pang, George Mardirossian |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
DNA damage medicine.medical_treatment Brachytherapy Biophysics General Physics and Astronomy Imaging phantom 030218 nuclear medicine & medical imaging 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Radiology Nuclear Medicine and imaging A-DNA Irradiation Gel electrophoresis Radiotherapy Dosage General Medicine Magnetic Resonance Imaging Radiation therapy Magnetic Fields chemistry 030220 oncology & carcinogenesis DNA Biomedical engineering DNA Damage |
| Zdroj: | Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB). 76 |
| ISSN: | 1724-191X |
| Popis: | Purpose Some studies have suggested that the presence of a static magnetic field (SMF) during irradiation alters biological damage. Since MRI-guided radiotherapy is becoming increasingly common, we constructed a DNA-based detector to assess the effect of a 1.5 T SMF on DNA damage during high dose rate (HDR) brachytherapy irradiation. Methods Block phantoms containing a small cavity for the placement of plasmid DNA (pBR322) samples were 3-D printed with biocompatible tissue equivalent material. The phantom was CT scanned and an HDR brachytherapy treatment plan was designed to deliver 20 Gy and 30 Gy doses to the DNA samples in the presence and absence of a 1.5 T SMF. Relative yields of single- and double-strand breaks (SSBs and DSBs, respectively) were computed from gel electrophoresis images of the DNA band intensities and averaged over sample sizes ranging from 12 to 30. Radiation dose was also measured in the presence and absence of the 1.5 T SMF using GafChromic™ EBT3 film placed in the coronal, sagittal, and axial planes. Results The average yield of DNA with SSBs and DSBs in the presence and absence of the SMF showed no statistically significant differences (all p ≥ 0.17). Differences in the net optical densities of the EBT3 films for each plane were within experimental uncertainty, suggesting no dose difference in the presence and absence of the SMF. Conclusions HDR irradiation in the presence of the 1.5 T SMF did not alter dose deposition to the DNA cavity nor change SSB and DSB DNA damage. |
| Databáze: | OpenAIRE |
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