High capacity in G protein-coupled receptor signaling
| Autor: | Alexey Koval, Sven Bergmann, Amiran Keshelava, Vladimir L. Katanaev, Mikhail Kryuchkov, Gonzalo P. Solis, Micha Hersch |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Agonist Calcium/metabolism medicine.drug_class Science General Physics and Astronomy Muscarinic Agonists General Biochemistry Genetics and Molecular Biology Article Receptors G-Protein-Coupled Cell Line 03 medical and health sciences Channel capacity Muscarinic acetylcholine receptor Receptors Extracellular medicine Humans Acetylcholine/pharmacology Cell Membrane/metabolism HEK293 Cells Muscarinic Agonists/pharmacology Receptor Muscarinic M3/metabolism Receptors G-Protein-Coupled/agonists Receptors G-Protein-Coupled/metabolism Signal Transduction/physiology Receptor lcsh:Science G protein-coupled receptor Receptor Muscarinic M3 Multidisciplinary Chemistry HEK 293 cells Cell Membrane Muscarinic M3/metabolism General Chemistry G Protein-Coupled Receptor Signaling Acetylcholine Cell biology 030104 developmental biology G-Protein-Coupled/agonists/metabolism lcsh:Q Calcium Signal Transduction |
| Zdroj: | Nature Communications, Vol 9, Iss 1, Pp 1-8 (2018) Nature Communications, Vol. 9, No 1 (2018) P. 876 Nature Communications Nature communications, vol. 9, no. 1, pp. 876 |
| ISSN: | 2041-1723 |
| Popis: | G protein-coupled receptors (GPCRs) constitute a large family of receptors that activate intracellular signaling pathways upon detecting specific extracellular ligands. While many aspects of GPCR signaling have been uncovered through decades of studies, some fundamental properties, like its channel capacity—a measure of how much information a given transmission system can reliably transduce—are still debated. Previous studies concluded that GPCRs in individual cells could transmit around one bit of information about the concentration of the ligands, allowing only for a reliable on or off response. Using muscarinic receptor-induced calcium response measured in individual cells upon repeated stimulation, we show that GPCR signaling systems possess a significantly higher capacity. We estimate the channel capacity of this system to be above two, implying that at least four concentration levels of the agonist can be distinguished reliably. These findings shed light on the basic principles of GPCR signaling. G protein-coupled receptors (GPCRs) activate intracellular signalling pathways upon extracellular stimulation. Here authors record single cell responses of GPCR signalling which allows the direct estimation of its channel capacity for each cell along with the reproducibility of its response. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|