Residual Cajal bodies in coilin knockout mice fail to recruit Sm snRNPs and SMN, the spinal muscular atrophy gene product
| Autor: | Erica Y. Jacobs, Jennifer J. Rossire, Karen E. Tucker, Miguel Lafarga, Maria T. Berciano, A. Gregory Matera, Karl B. Shpargel, Edward K. L. Chan, Ronald A. Conlon, David F. LePage |
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| Rok vydání: | 2001 |
| Předmět: |
Chromosomal Proteins
Non-Histone RNA Splicing Recombinant Fusion Proteins Green Fluorescent Proteins Gene Expression Coiled Bodies Nerve Tissue Proteins Biology Autoantigens snRNP Core Proteins Article Cell Line Mice 03 medical and health sciences 0302 clinical medicine SMN Complex Proteins Animals snRNP RNA Messenger coilin SMN SMA snRNPs nuclear organization Histone locus body Cyclic AMP Response Element-Binding Protein Fetal Viability Research Articles 030304 developmental biology SnRNP Biogenesis Mice Knockout Fibrillarin 0303 health sciences SnRNP Core Proteins Homozygote Nuclear Proteins RNA-Binding Proteins Cell Biology Blotting Northern Phosphoproteins Ribonucleoproteins Small Nuclear Molecular biology Survival Rate Luminescent Proteins Cajal body Organ Specificity Gene Targeting Coilin Cell Nucleolus 030217 neurology & neurosurgery |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Cajal bodies (CBs) are nuclear suborganelles involved in the biogenesis of small nuclear ribonucleoproteins (snRNPs). In addition to snRNPs, they are highly enriched in basal transcription and cell cycle factors, the nucleolar proteins fibrillarin (Fb) and Nopp140 (Nopp), the survival motor neuron (SMN) protein complex, and the CB marker protein, p80 coilin. We report the generation of knockout mice lacking the COOH-terminal 487 amino acids of coilin. Northern and Western blot analyses demonstrate that we have successfully removed the full-length coilin protein from the knockout animals. Some homozygous mutant animals are viable, but their numbers are reduced significantly when crossed to inbred backgrounds. Analysis of tissues and cell lines from mutant animals reveals the presence of extranucleolar foci that contain Fb and Nopp but not other typical nucleolar markers. These so-called “residual” CBs neither condense Sm proteins nor recruit members of the SMN protein complex. Transient expression of wild-type mouse coilin in knockout cells results in formation of CBs and restores these missing epitopes. Our data demonstrate that full-length coilin is essential for proper formation and/or maintenance of CBs and that recruitment of snRNP and SMN complex proteins to these nuclear subdomains requires sequences within the coilin COOH terminus. |
| Databáze: | OpenAIRE |
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