MIRA analysis of RARβ2 gene methylation in DNA circulating in the blood in lung cancer
| Autor: | Anastasia A. Ponomaryova, E. Yu. Rykova, Tatyana L. Azhikina, Pavel P. Laktionov, V. V. Vlasov, Nadezhda V. Cherdyntseva, K. Yu. Kapitskaya |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Lung Neoplasms Receptors Retinoic Acid Biology General Biochemistry Genetics and Molecular Biology Epigenesis Genetic chemistry.chemical_compound Carcinoma Non-Small-Cell Lung medicine Biomarkers Tumor Humans Sulfites Cancer epigenetics Epigenetics Gene Aged Cancer General Medicine Methylation DNA Neoplasm DNA Methylation Middle Aged medicine.disease Molecular biology chemistry CpG site Case-Control Studies DNA methylation Biological Assay CpG Islands Female DNA |
| Zdroj: | Bulletin of experimental biology and medicine. 157(4) |
| ISSN: | 1573-8221 |
| Popis: | Analysis of DNA epigenetic mutations in the blood circulating DNA is a prospective trend for creation of noninvasive methods for the diagnosis and treatment effi ciency monitoring in cancer. The methylation status of target genes in circulating DNA was evaluated by methods based on preliminary bisulfi te conversion of DNA. We used a different approach based on selection of hypermethylated sequences of circulating DNA by means of DNA-methyl-binding protein (methylated CpG island recovery assay, MIRA). Methylation was evaluated for RARβ2 tumor suppression gene in circulating DNA in lung cancer and a trend was detected to higher methylation of this gene in the patients in comparison with healthy donors. |
| Databáze: | OpenAIRE |
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