Proteoglycan biosynthesis by rabbit articular chondrocytes treated withd-penicillamine
Autor: | M. Bouakka, M. Langris, R. Beliard, Loyau G, Pujol Jp, P. Legendre, J. Bocquet |
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Rok vydání: | 1988 |
Předmět: |
Cartilage
Articular Immunology Molecular Conformation Biology Toxicology Chondrocyte Glycosaminoglycan chemistry.chemical_compound Hyaluronic acid medicine Animals Chondroitin Pharmacology (medical) Cells Cultured Glycosaminoglycans Pharmacology Lagomorpha Cartilage Penicillamine biology.organism_classification Molecular biology Extracellular Matrix Molecular Weight carbohydrates (lipids) medicine.anatomical_structure Proteoglycan chemistry Biochemistry Chromatography Gel biology.protein Proteoglycans Rabbits medicine.drug |
Zdroj: | Agents and Actions. 25:171-181 |
ISSN: | 1420-908X 0065-4299 |
DOI: | 10.1007/bf01969109 |
Popis: | Rabbit articular chondrocytes in confluent monolayer cultures were treated with D-Penicillamine (D-Pen) during 3 or 5 days. The [35S]-sulfate incorporation in neosynthesized proteoglycans was not modified by D-Pen doses ranging from 50 to 800 micrograms/ml. After treatment during 5 days with D-Pen concentrations of 50 or 400 micrograms/ml, the chemical characteristics of proteoglycans from medium and cell-layer were determined. The aggregation capacity of proteoglycans from medium, the monomer molecular size, the glycosaminoglycan chain length and the relative rates of the different glycosaminoglycans (chondroitins, chondroitin 6-sulfate, chondroitin 4-sulfate, hyaluronic acid) remained unchanged. These results suggest that D-Pen does not alter some of the cartilage mechanical properties due to the presence of proteoglycans. |
Databáze: | OpenAIRE |
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