Rice Husk as an Inexpensive Renewable Immobilization Carrier for Biocatalysts Employed in the Food, Cosmetic and Polymer Sectors
| Autor: | Francesca Vita, Lorenzo Del Terra, Simone Lotteria, Marco Cespugli, Luciano Navarini, Valerio Ferrario, Marina Zweyer, Lucia Gardossi, Cynthia Ebert, Giovanna Baldini, Valentina Lonzarich |
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| Přispěvatelé: | Cespugli, Marco, Simone, Lotteria, Navarini, Luciano, Lonzarich, Valentina, DEL TERRA, Lorenzo, Vita, Francesca, Zweyer, Marina, Baldini, Giovanna, Ferrario, Valerio, Ebert, Cynthia, Gardossi, Lucia |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
aspartase
010402 general chemistry CaLB lcsh:Chemical technology 01 natural sciences Husk Catalysis covalent immobilization of enzyme lcsh:Chemistry chemistry.chemical_compound covalent immobilization of enzymes rice husk polycondensation itaconic acid acrylamide aspartase renewable carriers biomass lcsh:TP1-1185 Itaconic acid renewable carriers Physical and Theoretical Chemistry chemistry.chemical_classification Aqueous solution biology biomass 010405 organic chemistry food and beverages Epoxy Polymer biology.organism_classification 0104 chemical sciences chemistry Chemical engineering lcsh:QD1-999 Biocatalysis visual_art visual_art.visual_art_medium acrylamide Surface modification Candida antarctica |
| Zdroj: | Catalysts, Vol 8, Iss 10, p 471 (2018) Catalysts Volume 8 Issue 10 |
| ISSN: | 2073-4344 |
| Popis: | The high cost and environmental impact of fossil-based organic carriers represent a critical bottleneck to their use in large-scale industrial processes. The present study demonstrates the applicability of rice husk as inexpensive renewable carrier for the immobilization of enzymes applicable sectors where the covalent anchorage of the protein is a pre-requisite for preventing protein contamination while assuring the recyclability. Rice husk was oxidized and then functionalized with a di-amino spacer. The morphological characterization shed light on the properties that affect the functionalization processes. Lipase B from Candida antarctica (CaLB) and two commercial asparaginases were immobilized covalently achieving higher immobilization yield than previously reported. All enzymes were immobilized also on commercial epoxy methacrylic resins and the CaLB immobilized on rice husk demonstrated a higher efficiency in the solvent-free polycondensation of dimethylitaconate. CaLB on rice husk appears particularly suitable for applications in highly viscous processes because of the unusual combination of its low density and remarkable mechanical robustness. In the case of the two asparaginases, the biocatalyst immobilized on rice husk performed in aqueous solution at least as efficiently as the enzyme immobilized on methacrylic resins, although the rice husk loaded a lower amount of protein. |
| Databáze: | OpenAIRE |
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