Correction to: A multiplex marker set for microsatellite typing and sexing of sooty terns Onychoprion fuscatus
Autor: | Deborah A. Dawson, Lucy J. H. Garrett, Gavin J. Horsburgh, S. James Reynolds |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Male Conservation of Natural Resources Onychoprion fuscatus Genotyping Techniques Population genetics Sex-typing lcsh:Medicine Conservation Sexing General Biochemistry Genetics and Molecular Biology Set (abstract data type) 03 medical and health sciences Charadriiformes 0302 clinical medicine Animals Multiplex 030212 general & internal medicine Typing lcsh:Science (General) lcsh:QH301-705.5 Atlantic Ocean Islands Polymorphism Genetic Geography lcsh:R Correction Colonial seabird General Medicine Sequence Analysis DNA Genealogy Ascension island Research Note PCR 030104 developmental biology Genetics Population lcsh:Biology (General) Genetic Loci Microsatellite Female Relatedness lcsh:Q1-390 Microsatellite loci Microsatellite Repeats |
Zdroj: | BMC Research Notes BMC Research Notes, Vol 11, Iss 1, Pp 1-1 (2018) |
ISSN: | 1756-0500 |
Popis: | Objectives Seabirds have suffered dramatic population declines in recent decades with one such species being the sooty tern Onychoprion fuscatus. An urgent call to re-assess their conservation status has been made given that some populations, such as the one on Ascension Island, South Atlantic, have declined by over 80% in three generations. Little is known about their population genetics, which would aid conservation management through understanding ecological processes and vulnerability to environmental change. We developed a multiplex microsatellite marker set for sooty terns including sex-typing markers to assist population genetics studies. Results Fifty microsatellite loci were isolated and tested in 23 individuals from Ascension Island. Thirty-one were polymorphic and displayed between 4 and 20 alleles. Three loci were Z-linked and two autosomal loci deviated from Hardy–Weinberg equilibrium. The remaining 26 autosomal loci together with three sex-typing makers were optimised in seven polymerase chain reaction plexes. These 26 highly polymorphic markers will be useful for understanding genetic structure of the Ascension Island population and the species as a whole. Combining these with recently developed microsatellite markers isolated from Indian Ocean birds will allow for assessment of global population structure and genetic diversity. Electronic supplementary material The online version of this article (10.1186/s13104-017-3084-9) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
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