Multiliter Production and Immunochemical Cross‐Reactivity of Plant Tissue Culture Antigens
| Autor: | Malcolm N. Blumenthal, W. L. Wu, E. J. Staba |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Ragweed
Immunodiffusion Plant tissue culture Size-exclusion chromatography Pharmaceutical Science Cross Reactions medicine.disease_cause Cross-reactivity chemistry.chemical_compound Tissue culture Antigen Antibody Specificity Culture Techniques Botany medicine Animals Humans Food science Antigens Ammonium sulfate precipitation Plant Proteins Skin Tests biology Immunochemistry food and beverages Allergens Plants biology.organism_classification Dextran chemistry Rabbits |
| Zdroj: | Journal of Pharmaceutical Sciences. 65:102-105 |
| ISSN: | 0022-3549 |
| DOI: | 10.1002/jps.2600650122 |
| Popis: | Cells of short ragweed suspension culture were successfully propagated in a multiliter fermentor. Proteins were isolated from tissue cultures (short ragweed, cantaloupe, marigold, and Ammi) and plants (short ragweed pollen, cantaloupe fruit, and marigold flowers) by ammonium sulfate precipitation, cross‐linked dextran gel filtration, and anion‐exchange chromatography. Some fractions elicited allergenic responses in ragweed‐sensitive patients but not in ragweed‐nonsensitive control patients. Double‐immuno‐diffusion analysis of these fractions showed their nonidentity with short ragweed antigen E. |
| Databáze: | OpenAIRE |
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