X-Linked Huwe1 Is Essential for Oocyte Maturation and Preimplantation Embryo Development
| Autor: | Alaa Eisa, Ning Kon, Manabu Kurokawa, William W. Feng, Katherine J. Crawford, Scott Bang, Wei Gu, Wendy A. Wells, Souvik Dey, Srinivasan Vijayaraghavan, Emily M. Murphy, Lisa M. Mehlmann |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
02 engineering and technology Female reproductive system Article Andrology 03 medical and health sciences Human fertilization medicine lcsh:Science Multidisciplinary biology Embryogenesis Female infertility Embryo Cell Biology 021001 nanoscience & nanotechnology Antral follicle medicine.disease Oocyte Ubiquitin ligase 030104 developmental biology medicine.anatomical_structure biology.protein lcsh:Q 0210 nano-technology Developmental Biology |
| Zdroj: | iScience, Vol 23, Iss 9, Pp 101523-(2020) iScience |
| ISSN: | 2589-0042 |
| Popis: | Summary HUWE1 is a HECT-domain ubiquitin E3 ligase expressed in various tissues. Although HUWE1 is known to promote degradation of the tumor suppressor p53, given a growing list of its substrates, in vivo functions of HUWE1 remain elusive. Here, we investigated the role of HUWE1 in the female reproductive system. Homozygous deletion of Huwe1 in mouse oocytes of primary follicles caused oocyte death and female infertility, whereas acute depletion of HUWE1 protein by Trim-Away technology did not impact oocytes from antral follicles. Interestingly, oocytes from Huwe1 heterozygous females matured and fertilized normally, but the majority of embryos that lacked maternal Huwe1 were arrested at the morula stage after fertilization. Consequently, Huwe1 heterozygous females only produced wild-type pups. Concomitant knockout of p53 did not recover fertility of the Huwe1 knockout females. These findings make HUWE1 a unique and critical maternal factor indispensable for maintaining the quality of oocytes and embryos. Graphical Abstract Highlights • Oocyte-specific Huwe1 KO leads to female infertility in mice • Homozygous, but not heterozygous, Huwe1 KO kills oocytes before the GV stage • Maternal Huwe1 is also required for preimplantation embryonic development • Defects in oocyte and embryonic development of Huwe1 KO mice are independent of p53 Cell Biology; Developmental Biology |
| Databáze: | OpenAIRE |
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