An Epitope Delivery System for Use with Recombinant Mycobacteria
| Autor: | Charlotte Hetzel, Jonathan B. Cooper, Nanna M. Kristensen, Riny Janssen, Jonathan R. Lamb, Douglas B. Young, Sarah J. Ely, Karen Bunting, J.E.R. Thole |
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| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
CD4-Positive T-Lymphocytes
Recombinant Fusion Proteins Immunology Molecular Sequence Data Epitopes T-Lymphocyte Gene Expression CD8-Positive T-Lymphocytes Microbiology Epitope law.invention Mycobacterium Interferon-gamma Mice Antigen Bacterial Proteins law Animals Amino Acid Sequence Antigens Dermatophagoides Cloning Molecular Glycoproteins Recombination Genetic Mycobacterium bovis Antigens Bacterial biology Superoxide Dismutase Immunogenicity biology.organism_classification Fusion protein Virology Mice Inbred C57BL Infectious Diseases Microbial Immunity and Vaccines Recombinant DNA Parasitology Female Mycobacterium vaccae |
| Popis: | We have developed a novel epitope delivery system based on the insertion of peptides within a permissive loop of a bacterial superoxide dismutase molecule. This system allowed high-level expression of heterologous peptides in two mycobacterial vaccine strains, Mycobacterium bovis bacille Calmette- Guerin (BCG) and Mycobacterium vaccae. The broader application of the system was analyzed by preparation of constructs containing peptide epitopes from a range of infectious agents and allergens. We report detailed characterization of the immunogenicity of one such construct, in which an epitope from the Der p1 house dust mite allergen was expressed in M. vaccae. The construct was able to stimulate T-cell hybridomas specific for Der p1, and it induced peptide-specific gamma interferon responses when used to immunize naive mice. This novel expression system demonstrates new possibilities for the use of mycobacteria as vaccine delivery vehicles. Chemicals/CAS: Antigens, Bacterial; Antigens, Dermatophagoides; Bacterial Proteins; Epitopes, T-Lymphocyte; Glycoproteins; Interferon Type II, 82115-62-6; Recombinant Fusion Proteins; SodA protein, Bacteria; Superoxide Dismutase, EC 1.15.1.1 |
| Databáze: | OpenAIRE |
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