Molecular diversity of Proteus mirabilis isolates producing extended-spectrum β-lactamases in a French university hospital
| Autor: | M. Biendo, D. Thomas, F. Hamdad-Daoudi, G. Laurans, Sandrine Castelain, F. Rousseau, B. Canarelli, F. Eb |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Microbiology (medical)
Male β-Lactamases Urine beta-Lactamases Microbiology Hospitals University resistance Drug Resistance Bacterial Pulsed-field gel electrophoresis Prevalence Humans Typing Proteus mirabilis TEM-type ESBLs biology Molecular epidemiology Isoelectric focusing β lactamases Incidence (epidemiology) Genetic Variation General Medicine PFGE University hospital biology.organism_classification Anti-Bacterial Agents Community-Acquired Infections Infectious Diseases ESBLs Female France Proteus Infections |
| Zdroj: | Clinical Microbiology and Infection. (5):395-401 |
| ISSN: | 1198-743X |
| DOI: | 10.1111/j.1469-0691.2005.01147.x |
| Popis: | Between February 1997 and December 2002, 3340 hospitalised patients yielded samples positive for Proteus mirabilis , of whom 45 (1.3%) were colonised/infected by P. mirabilis producing extended-spectrum β-lactamases (ESBLs). The gross incidence of patients colonised/infected by ESBL-producing P. mirabilis was 1.61/10 5 days of hospitalisation, with 20% of isolates being collected from patients in urology wards, most frequently (53.3%) from urine samples. Seventeen (37.7%) of the 43 isolates were obtained from samples collected within 48 h of hospitalisation, indicating that they were community-acquired. Isoelectric focusing assays and sequencing identified the TEM-24, TEM-92 and TEM-52 ESBLs. Pulsed-field gel electrophoresis revealed eight pulsotypes (I–VIII), with the two most common pulsotypes, IV and VI, comprising ten (23.3%) and 12 (26.6%) isolates, respectively. These pulsotypes were considered to represent epidemic strains and spread in various wards of the hospital. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|