Holder pasteurization impacts the proteolysis, lipolysis and disintegration of human milk under in vitro dynamic term newborn digestion
| Autor: | Florence Rousseau, Emelyne Dirson, Amélie Deglaire, Claire Bourlieu, Olivia Ménard, Frédéric Carrière, Amandine Bellanger, Samira de Oliveira, Didier Dupont, Gwénaële Henry |
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| Přispěvatelé: | Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Service de Pédiatrie, Centre Hospitalier Universitaire [Rennes], Enzymologie interfaciale et de physiologie de la lipolyse (EIPL), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Proteolysis [SDV]Life Sciences [q-bio] Pasteurization law.invention 03 medical and health sciences law medicine Lipolysis Food science Milk Banks chemistry.chemical_classification 030109 nutrition & dietetics in vitro dynamic digestion biology medicine.diagnostic_test Lactoferrin Human milk Amino acid Infant formula Biochemistry chemistry term newborn biology.protein Holder pasteurization lipolysis Digestion Food Science |
| Zdroj: | Food Research International Food Research International, 2016, 88 part B, in press. ⟨10.1016/j.foodres.2015.11.022⟩ Food Research International, Elsevier, 2016, 88 part B, in press. ⟨10.1016/j.foodres.2015.11.022⟩ Food Research International, Elsevier, 2016, in press. ⟨10.1016/j.foodres.2015.11.022⟩ |
| ISSN: | 0963-9969 |
| DOI: | 10.1016/j.foodres.2015.11.022⟩ |
| Popis: | International audience; When the mother’s own human milk is unavailable or limited, pasteurized human milk from milk banks is preferentially administered instead of infant formula, especially for vulnerable hospitalized neonates. Holder pasteurization (62.5 °C, 30 min) may alter human milk composition and structure, which may modulate its digestive behavior. An in vitro dynamic system was set up to simulate the gastrointestinal digestion of term newborns in order to compare the kinetics of lipolysis, proteolysis and structural disintegration of raw versus pasteurized human milk. Human milk from 5 donors was pooled. Half of the pool was either administrated raw (RHM) or pasteurized (PHM). Digestions were conducted at least in duplicate for RHM and PHM. Heat-induced protein aggregation was observed in PHM. During gastric digestion, β-casein was proteolyzed significantly faster for PHM than for RHM (p < 0.05), whereas lactoferrin tended to be proteolyzed slower (p = 0.07) for PHM. Pasteurization selectively affected the intestinal release of some amino acids. At any time of the gastrointestinal digestion, the lipolysis of PHM was significantly lower than that of RHM, but no impact was observed on the profile of released fatty acids. RHM presented a structural destabilization after 60 min of gastric digestion, while there was no large variation for PHM. In the intestinal phase, the evolution of the particle sizes was rather similar. Overall, Holder pasteurization impacted the proteolysis, lipolysis and disintegration of human milk. However, this impact was limited and the physiologic and metabolic consequences remain to be investigated. |
| Databáze: | OpenAIRE |
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