Rapid detection of Salmonella in milk by electrochemical magneto-immunosensing
| Autor: | María Isabel Pividori, Susana Campoy, María Pilar Cortés, Susana Liébana, Anabel Lermo, Salvador Alegret |
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| Rok vydání: | 2009 |
| Předmět: |
Salmonella
Transducers Biomedical Engineering Biophysics Food Contamination Biosensing Techniques medicine.disease_cause Immunomagnetic separation Microbiology medicine Electrochemistry Animals Escherichia coli Electrodes Detection limit Chromatography biology Immunomagnetic Separation Pathogenic bacteria General Medicine Equipment Design biology.organism_classification Enterobacteriaceae Equipment Failure Analysis Milk Cattle Biosensor Bacteria Food Analysis Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 25(2) |
| ISSN: | 1873-4235 |
| Popis: | A very simple and rapid method for the detection of Salmonella in milk is reported. In this approach, the bacteria are captured and preconcentrated from milk samples with magnetic beads through an immunological reaction. A second polyclonal antibody labeled with peroxidase is used as serological confirmation with electrochemical detection based on a magneto-electrode. The ‘IMS/m-GEC electrochemical immunosensing’ approach shows a limit of detection of 5 × 10 3 and 7.5 × 10 3 CFU mL −1 in LB and in milk diluted 1/10 in LB broth, respectively, in 50 min without any pretreatment. If the skimmed-milk is preenriched for 6 h, the method is able to detect as low as 1.4 CFU mL −1 , while if it is preenriched for 8 h, as low as 0.108 × CFU mL −1 (2.7 × CFU in 25 g of milk, in 5 samples of 5 mL) are detected accordingly with the legislation. Moreover, the method is able to clearly distinguish between food pathogenic bacteria such as Salmonella and Escherichia coli . The features of this approach are discussed and compared with classical culture methods. |
| Databáze: | OpenAIRE |
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